APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:20000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
293T , NIH-3T3 , mouse brain , PC-12 , rat brain , E. coli M15 , Drosophila , yeast , zebrafish
Predict Reactivity
Rabbit, Sheep, Bovine, Cat, Dog, Chicken, Guinea pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1mg/ml(Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human GAPDH. The exact sequence is proprietary.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
G3PD antibody, GAPD antibody, MGC88685 antibody, GAPDH antibody, glyceraldehyde-3-phosphate dehydrogenase antibody, aging-associated gene 9 protein antibody, glyceraldehyde 3-phosphate dehydrogenase antibody, peptidyl-cysteine S-nitrosylase GAPDH antibody
Cellular Localization
Cytoplasm , perinuclear region , Membrane
Background
The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX627408 WB Image
Sample (30 µg of whole cell lysate)
A: Drosophila
10% SDS PAGE
GTX627408 diluted at 1:5000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX627408 IHC-P Image
GAPDH antibody [GT239] detects GAPDH protein at cytosol on U373 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U373 xenograft.
GAPDH antibody [GT239] (GTX627408) dilution: 1:200.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX627408 WB Image
GAPDH antibody [GT239] detects GAPDH protein by western blot analysis.
A. 30 µg whole zebrafish lysate/extract
10% SDS-PAGE
GAPDH antibody [GT239] (GTX627408) dilution: 1:500
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX627408 WB Image
GAPDH antibody [GT239] detects GAPDH protein by western blot analysis.
A. 30 µg yeast whole cell lysate/extract
10% SDS-PAGE
GAPDH antibody [GT239] (GTX627408) dilution: 1:10000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX627408 WB Image
GAPDH antibody [GT239] detects GAPDH protein by western blot analysis.
A. 30 µg 293T whole cell lysate/extract
B. 30 µg NIH-3T3 whole cell lysate/extract
C. 30 µg mouse brain lysate/extract
D. 30 µg PC-12 whole cell lysate/extract
E. 30 µg rat brain lysate/extract
10% SDS-PAGE
GAPDH antibody [GT239] (GTX627408) dilution: 1:10000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX627408 ICC/IF Image
GAPDH antibody [GT239] detects GAPDH protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: GAPDH stained by GAPDH antibody [GT239] (GTX627408) diluted at 1:500.Blue: Hoechst 33342 staining.
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GTX627408 WB Image
GAPDH antibody [GT239] detects GAPDH protein by western blot analysis.
A. 20 µg 293T whole cell lysate/extract
B. 10 µg 293T whole cell lysate/extract
C. 5 µg 293T whole cell lysate/extract
D. 1 µg 293T whole cell lysate/extract
10% SDS-PAGE
GAPDH antibody [GT239] (GTX627408) dilution: 1:10000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX627408 WB Image
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with (GTX627408) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX627408 WB Image
Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody [GT239] (GTX627408) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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REFERENCE
REVIEW
GAPDH antibody [GT239]
Cat No. GTX627408
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Rating
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( Average 4.5 based on 6 users reviews)
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Application
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Western Blot(WB)
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( Average 4.5 based on 6 users reviews)
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Date :
Anonymous submitted on 24-Apr-2017
Application Tested :
WB
Sample Species :
Rat
Sample :
Brain tissue
Amount used :
50 μg
Blocking :
3% BSA in TBST, 23°C, 1Hr
Primary Antibody :
1:10000, 4°C, overnightHr
Date :
Anonymous submitted on 30-Jun-2014
Application Tested :
WB
Sample Species :
Hu
Sample :
KATOIII cells, MKN45 cells
Amount used :
100μg
Blocking :
skim milk, 25°C, 1Hr
Primary Antibody :
1:5000, 4°C, overnightHr
Lane Description :
Lane1:KATOIII cells, Lane2:MKN45 cells
Date :
Anonymous submitted on 18-Apr-2014
Application Tested :
WB
Sample Species :
Hu
Sample :
SHSY-5Y cells
Amount used :
30μg
Blocking :
5% Skim milk in TBST, 25°C, 2Hr
Primary Antibody :
1:5000, 4°C, overnightHr
Date :
Anonymous submitted on 06-Mar-2014
Application Tested :
WB
Sample Species :
Rat
Sample :
PC12 cells
Amount used :
20μg
Blocking :
5% nonfat milk, 22°C, 0.5Hr
Primary Antibody :
1:1000, 22°C, 2Hr
Figure Note :
starreivew courtesy of Xiaobei(Laboratory of UCSD)
Date :
Anonymous submitted on 06-Feb-2012
Application Tested :
WB
Sample Species :
Hu
Sample :
293T cells
Amount used :
30μg
Blocking :
TBST with 5% milk, 25°C, 1Hr
Primary Antibody :
1:1000, 4°C, overnightHr
Date :
Anonymous submitted on 06-Feb-2012
Application Tested :
WB
Sample Species :
Hu
Sample :
M10 cells
Amount used :
40μg
Blocking :
TBST with 5% milk, 25°C, 1Hr
Primary Antibody :
1:5000, 25°C, 1Hr
