GeneTex
United States (US)

GAPDH antibody [GT239]

GTX627408_40846_WB_DP_18121411_845.jpg
GTX627408_41036_IHC_18121411_114.jpg
GTX627408_40846_WB_Z_18121411_991.jpg
GTX627408_41323_WB_yeast_18121411_243.jpg
GTX627408_41323_WB_multiple-species_18121411_201.jpg
GTX627408_43010_20180117_ICC_IF_18121411_218.jpg
GTX627408_41323_WB_sensitivity_18121411_744.jpg
GTX627408_41323_20171103_WB_18121411_628.jpg
GTX627408_41323_20161013_WB_E. coli_18121411_436.jpg

Cat No. GTX627408

Host Mouse
Clonality Monoclonal
Clone Name GT239
Isotype IgG2b
Application WB, ICC/IF, IHC-P, EMSA
Reactivity Human, Mouse, Rat, Zebrafish, Yeast, Drosophila, Pig, Monkey, E. coli
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
EMSA Assay dependent
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

293T , NIH-3T3 , mouse brain , PC-12 , rat brain , E. coli M15 , Drosophila , yeast , zebrafish

Predict Reactivity

Rabbit, Sheep, Bovine, Cat, Dog, Chicken, Guinea pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GAPDH. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

G3PD antibody, GAPD antibody, MGC88685 antibody, GAPDH antibody, glyceraldehyde-3-phosphate dehydrogenase antibody, aging-associated gene 9 protein antibody, glyceraldehyde 3-phosphate dehydrogenase antibody, peptidyl-cysteine S-nitrosylase GAPDH antibody

Cellular Localization

Cytoplasm , perinuclear region , Membrane

Background

The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX627408_40846_WB_DP_18121411_845.jpg

GTX627408 WB Image

Sample (30 µg of whole cell lysate)
A: Drosophila
10% SDS PAGE
GTX627408 diluted at 1:5000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX627408_41036_IHC_18121411_114.jpg

GTX627408 IHC-P Image

GAPDH antibody [GT239] detects GAPDH protein at cytosol on U373 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U373 xenograft.
GAPDH antibody [GT239] (GTX627408) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX627408_40846_WB_Z_18121411_991.jpg

GTX627408 WB Image

GAPDH antibody [GT239] detects GAPDH protein by western blot analysis.
A. 30 µg whole zebrafish lysate/extract
10% SDS-PAGE
GAPDH antibody [GT239] (GTX627408) dilution: 1:500
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX627408_41323_WB_yeast_18121411_243.jpg

GTX627408 WB Image

GAPDH antibody [GT239] detects GAPDH protein by western blot analysis.
A. 30 µg yeast whole cell lysate/extract
10% SDS-PAGE
GAPDH antibody [GT239] (GTX627408) dilution: 1:10000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX627408_41323_WB_multiple-species_18121411_201.jpg

GTX627408 WB Image

GAPDH antibody [GT239] detects GAPDH protein by western blot analysis.
A. 30 µg 293T whole cell lysate/extract
B. 30 µg NIH-3T3 whole cell lysate/extract
C. 30 µg mouse brain lysate/extract
D. 30 µg PC-12 whole cell lysate/extract
E. 30 µg rat brain lysate/extract
10% SDS-PAGE
GAPDH antibody [GT239] (GTX627408) dilution: 1:10000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX627408_43010_20180117_ICC_IF_18121411_218.jpg

GTX627408 ICC/IF Image

GAPDH antibody [GT239] detects GAPDH protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: GAPDH stained by GAPDH antibody [GT239] (GTX627408) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX627408_41323_WB_sensitivity_18121411_744.jpg

GTX627408 WB Image

GAPDH antibody [GT239] detects GAPDH protein by western blot analysis.
A. 20 µg 293T whole cell lysate/extract
B. 10 µg 293T whole cell lysate/extract
C. 5 µg 293T whole cell lysate/extract
D. 1 µg 293T whole cell lysate/extract
10% SDS-PAGE
GAPDH antibody [GT239] (GTX627408) dilution: 1:10000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX627408_41323_20171103_WB_18121411_628.jpg

GTX627408 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with (GTX627408) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX627408_41323_20161013_WB_E. coli_18121411_436.jpg

GTX627408 WB Image

Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody [GT239] (GTX627408) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE
REVIEW

GAPDH antibody [GT239] Cat No. GTX627408

Rating ( Average 4.5 based on 6 users reviews)
Application
Western Blot(WB) ( Average 4.5 based on 6 users reviews)
Date : Anonymous submitted on 24-Apr-2017
Score :
Application Tested : WB
Sample Species : Rat
Sample : Brain tissue
Amount used : 50 μg
Blocking : 3% BSA in TBST, 23°C, 1Hr
Primary Antibody : 1:10000, 4°C, overnightHr
Date : Anonymous submitted on 30-Jun-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : KATOIII cells, MKN45 cells
Amount used : 100μg
Blocking : skim milk, 25°C, 1Hr
Primary Antibody : 1:5000, 4°C, overnightHr
Lane Description : Lane1:KATOIII cells, Lane2:MKN45 cells
Date : Anonymous submitted on 18-Apr-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : SHSY-5Y cells
Amount used : 30μg
Blocking : 5% Skim milk in TBST, 25°C, 2Hr
Primary Antibody : 1:5000, 4°C, overnightHr
Date : Anonymous submitted on 06-Mar-2014
Score :
Application Tested : WB
Sample Species : Rat
Sample : PC12 cells
Amount used : 20μg
Blocking : 5% nonfat milk, 22°C, 0.5Hr
Primary Antibody : 1:1000, 22°C, 2Hr
Figure Note : starreivew courtesy of Xiaobei(Laboratory of UCSD)
Date : Anonymous submitted on 06-Feb-2012
Score :
Application Tested : WB
Sample Species : Hu
Sample : 293T cells
Amount used : 30μg
Blocking : TBST with 5% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, overnightHr
Date : Anonymous submitted on 06-Feb-2012
Score :
Application Tested : WB
Sample Species : Hu
Sample : M10 cells
Amount used : 40μg
Blocking : TBST with 5% milk, 25°C, 1Hr
Primary Antibody : 1:5000, 25°C, 1Hr
Application Reference
SDS
PBS.pdf
Package List Price ($)
$ 319
$ 169