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GAPDH antibody [N2C1], Internal

Anti-GAPDH antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX100283
Anti-GAPDH antibody [N2C1], Internal used in Western Blot (WB). GTX100283
Anti-GAPDH antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100283
Anti-GAPDH antibody [N2C1], Internal used in Western Blot (WB). GTX100283

Cat No. GTX100283

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

A431 , H1299 , HeLa , HepG2 , Neuro2A , BCL-1 , Raw264.7 , *SH-SY5Y

Predict Reactivity

Rat, Rabbit, Cat, Dog, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human GAPDH. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

glyceraldehyde-3-phosphate dehydrogenase,G3PD,GAPD,HEL-S-162eP

Cellular Localization

Cytoplasm , perinuclear region , Membrane

Background

The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-GAPDH antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX100283

GTX100283 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using GAPDH(GTX100283) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GAPDH antibody [N2C1], Internal used in Western Blot (WB). GTX100283

GTX100283 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
10% SDS PAGE
GAPDH antibody
GTX100283 diluted at 1:1000

Anti-GAPDH antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100283

GTX100283 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using GAPDH(GTX100283) antibody at 1:100 dilution.

Anti-GAPDH antibody [N2C1], Internal used in Western Blot (WB). GTX100283

GTX100283 WB Image

GAPDH antibody [N2C1], Internal detects GAPDH protein by Western blot analysis.
A. 30 µg Neuro2A whole cell lysate/extract
B. 30 µg BCL-1 whole cell lysate/extract
C. 30 µg Raw264.7 whole cell lysate/extract
10 % SDS-PAGE
GAPDH antibody [N2C1], Internal (GTX100283) dilution: 1:2000

REFERENCE
REVIEW

GAPDH antibody [N2C1], Internal Cat No. GTX100283

Rating ( Average 4 based on 1 users reviews)
Application
Western Blot(WB) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 16-Jan-2012
Score :
Application Tested : WB
Sample Species : Hu
Sample : SH-SY5Y cells
Amount used : 25μg
Blocking : 5% non-fat milk, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169