GeneTex
United States (US)

GAPDH antibody [N2C1], Internal

GTX100283_39443_IHC_19011506_675.jpg
GTX100283_WB_19011506_448.jpg
GTX100283_IFA_19011506_670.jpg
GTX100283_39443_WB_M_19011506_839.jpg

Cat No. GTX100283

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

A431 , H1299 , HeLa , HepG2 , Neuro2A , BCL-1 , Raw264.7 , *SH-SY5Y

Predict Reactivity

Rat, Rabbit, Cat, Dog, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human GAPDH. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

G3PD antibody, GAPD antibody, MGC88685 antibody, GAPDH antibody, glyceraldehyde-3-phosphate dehydrogenase antibody, aging-associated gene 9 protein antibody, glyceraldehyde 3-phosphate dehydrogenase antibody, peptidyl-cysteine S-nitrosylase GAPDH antibody

Cellular Localization

Cytoplasm , perinuclear region , Membrane

Background

The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100283_39443_IHC_19011506_675.jpg

GTX100283 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using GAPDH(GTX100283) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100283_WB_19011506_448.jpg

GTX100283 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
10% SDS PAGE
GAPDH antibody
GTX100283 diluted at 1:1000

GTX100283_IFA_19011506_670.jpg

GTX100283 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using GAPDH(GTX100283) antibody at 1:100 dilution.

GTX100283_39443_WB_M_19011506_839.jpg

GTX100283 WB Image

GAPDH antibody [N2C1], Internal detects GAPDH protein by Western blot analysis.
A. 30 µg Neuro2A whole cell lysate/extract
B. 30 µg BCL-1 whole cell lysate/extract
C. 30 µg Raw264.7 whole cell lysate/extract
10 % SDS-PAGE
GAPDH antibody [N2C1], Internal (GTX100283) dilution: 1:2000

REFERENCE
REVIEW

GAPDH antibody [N2C1], Internal Cat No. GTX100283

Rating ( Average 4 based on 1 users reviews)
Application
Western Blot(WB) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 16-Jan-2012
Score :
Application Tested : WB
Sample Species : Hu
Sample : SH-SY5Y cells
Amount used : 25μg
Blocking : 5% non-fat milk, 1Hr
Primary Antibody : 1:1000, 4°C, overnightHr
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169