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GAPDH antibody [RM114]

Anti-GAPDH antibody [RM114] used in Western Blot (WB). GTX33615
Anti-GAPDH antibody [RM114] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33615

Cat No. GTX33615

Host

Rabbit

Clonality

Monoclonal

Clone Name

RM114

Isotype

IgG

Application

WB, ICC/IF, FACS, IP, ELISA, IHC

Reactivity

Human
Package
100 μl ($319)

APPLICATION

Application Note

Western Blot (WB): 1:1000 dilution, Immunoprecipitation (IP): 1:200 dilution, Chromatin IP (ChIP): 1:200 dilution, Immunocytochemistry (ICC): 1:200 dilution, Immunohistochemistry (IHC): 1:200 dilution.

Calculated MW

36 kDa. ( Note )

Specificity/Sensitivity

This antibody reacts to GAPDH.

PROPERTIES

Form

Liquid

Buffer

50% Glycerol/PBS with 1% BSA and 0.09% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Immunogen

A peptide corresponding to the C-terminus of GAPDH

Purification

Protein A affinity purified from an animal origin-free culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Glyceraldehyde-3-Phosphate Dehydrogenase , G3Pd , Gapd , Hel-S-162Ep , Gapdh

Background

This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]

Database

Research Area

DATA IMAGES

Anti-GAPDH antibody [RM114] used in Western Blot (WB). GTX33615

GTX33615 WB Image

Western blot of A431 cells, using anti-GAPDH Clone RM114 at 1/1000 dilution, showed a band ofGAPDH at the predicted MW (~36 kDa).

Anti-GAPDH antibody [RM114] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33615

GTX33615 ICC/IF Image

Immunocytochemical staining of HeLa cells, using anti-GAPDH Clone RM114 at 1/200 dilution (red). Nuclei have been labeled with DAPI (blue).

REFERENCE

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REVIEW

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Package List Price ($)
$ 319