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GAPDH antibody [RM114]

Anti-GAPDH antibody [RM114] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33615
Anti-GAPDH antibody [RM114] used in Western Blot (WB). GTX33615

Cat No. GTX33615

Host

Rabbit

Clonality

Monoclonal

Clone Name

RM114

Isotype

IgG

Application

WB, ICC/IF, FACS, IP, ELISA, IHC

Reactivity

Human, Mouse, Rat
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000
ICC/IF 1:200
FACS Assay dependent
IP 1:200
ELISA Assay dependent
IHC 1:200
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 0.09% sodium azide, 50% Glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A peptide corresponding to the C-terminus of GAPDH

Purification

Protein A purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

G3PD,GAPD,GAPDH,HELS162eP,glyceraldehyde3phosphate dehydrogenase,glyceraldehyde-3-phosphate dehydrogenase

Cellular Localization

Cytoplasm,Cytosol,Nucleus,Perinuclear region,Membrane,Cytoskeleton

Background

This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]

Database

Research Area

DATA IMAGES

Anti-GAPDH antibody [RM114] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33615

GTX33615 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX33615 GAPDH antibody [RM114].
Red : Primary antibody
Green : Actin
Blue : DAPI
Dilution : 1:200

Anti-GAPDH antibody [RM114] used in Western Blot (WB). GTX33615

GTX33615 WB Image

WB analysis of A431 cell lysates using GTX33615 GAPDH antibody [RM114].
Dilution : 1:1000

REFERENCE

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REVIEW

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