Western Blot (WB): 1:1000 dilution, Immunoprecipitation (IP): 1:200 dilution, Chromatin IP (ChIP): 1:200 dilution, Immunocytochemistry (ICC): 1:200 dilution, Immunohistochemistry (IHC): 1:200 dilution.
This antibody reacts to GAPDH.
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
A peptide corresponding to the C-terminus of GAPDH
Protein A affinity purified from an animal origin-free culture supernatant
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Glyceraldehyde-3-Phosphate Dehydrogenase , G3Pd , Gapd , Hel-S-162Ep , Gapdh
This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]
GTX33615 WB Image
Western blot of A431 cells, using anti-GAPDH Clone RM114 at 1/1000 dilution, showed a band ofGAPDH at the predicted MW (~36 kDa).
GTX33615 ICC/IF Image
Immunocytochemical staining of HeLa cells, using anti-GAPDH Clone RM114 at 1/200 dilution (red). Nuclei have been labeled with DAPI (blue).