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GAPDH antibody - VetSignal

Anti-GAPDH antibody - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX135012
Anti-GAPDH antibody - VetSignal used in Western Blot (WB). GTX135012
Anti-GAPDH antibody - VetSignal used in Western Blot (WB). GTX135012

Cat No. GTX135012

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Cat, Dog
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Positive Control

MDCK

Predict Reactivity

Human, Mouse, Rat, Zebrafish, Rabbit, Sheep, Bovine, Chicken, Guinea pig, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Concentration

0.14 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Recombinant protein corresponding to dog GAPDH.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Database

Research Area

DATA IMAGES
Anti-GAPDH antibody - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX135012

GTX135012 IHC-P Image

GAPDH antibody detects GAPDH protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog tongue.
GAPDH stained by GAPDH antibody (GTX135012) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GAPDH antibody - VetSignal used in Western Blot (WB). GTX135012

GTX135012 WB Image

Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody (GTX135012) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GAPDH antibody - VetSignal used in Western Blot (WB). GTX135012

GTX135012 WB Image

Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody (GTX135012) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319