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GAPDH antibody

Anti-GAPDH antibody used in Immunoprecipitation (IP). GTX100118
Anti-GAPDH antibody used in Western Blot (WB). GTX100118
Anti-GAPDH antibody used in Western Blot (WB). GTX100118
Anti-GAPDH antibody used in Western Blot (WB). GTX100118
Anti-GAPDH antibody used in IHC (Paraffin sections) (IHC-P). GTX100118
Anti-GAPDH antibody used in IHC (Paraffin sections) (IHC-P). GTX100118
Anti-GAPDH antibody used in IHC (Paraffin sections) (IHC-P). GTX100118
Anti-GAPDH antibody used in Western Blot (WB). GTX100118
Anti-GAPDH antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100118
Anti-GAPDH antibody used in Western Blot (WB). GTX100118
Anti-GAPDH antibody used in Western Blot (WB). GTX100118
Anti-GAPDH antibody used in Western Blot (WB). GTX100118
Anti-GAPDH antibody used in Western Blot (WB). GTX100118
Anti-GAPDH antibody used in Western Blot (WB). GTX100118
Anti-GAPDH antibody used in Western Blot (WB). GTX100118
Anti-GAPDH antibody used in Western Blot (WB). GTX100118

Cat No. GTX100118

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse, Rat, Zebrafish, Yeast, Rabbit, Drosophila, Bovine, Dog, Hamster, Chicken, Pig, Monkey, E. coli, Mosquito, Nematode, Pika, Fish, Bacteria
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:100000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2 , E.coli M15 , *SH-SY5Y , *A549 , *U2OS , *H9C2 , *A7R5 , *mouse skin tissue

Predict Reactivity

Sheep, Cat, Guinea pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.69 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GAPDH. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

glyceraldehyde-3-phosphate dehydrogenase , G3PD , GAPD , HEL-S-162eP

Cellular Localization

Cytoplasm , perinuclear region , Membrane

Background

The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-GAPDH antibody used in Immunoprecipitation (IP). GTX100118

GTX100118 IP Image

Immunoprecipitation of GAPDH protein from 293T whole cell extracts using 5 μg of GAPDH antibody (GTX100118).
Western blot analysis was performed using GAPDH antibody (GTX100118).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-GAPDH antibody used in Western Blot (WB). GTX100118

GTX100118 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody (GTX100118) diluted at 1:6000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GAPDH antibody used in Western Blot (WB). GTX100118

GTX100118 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody (GTX100118) diluted at 1:5000.

Anti-GAPDH antibody used in Western Blot (WB). GTX100118

GTX100118 WB Image

GAPDH antibody detects GAPDH protein by western blot analysis.
A. 30 µg PC-12 whole cell lysate/extract
B. 30 µg Rat2 whole cell lysate/extract
10% SDS-PAGE
GAPDH antibody (GTX100118) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GAPDH antibody used in IHC (Paraffin sections) (IHC-P). GTX100118

GTX100118 IHC-P Image

Immunohistochemical analysis of human salivary gland cancer, using GAPDH(GTX100118) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GAPDH antibody used in IHC (Paraffin sections) (IHC-P). GTX100118

GTX100118 IHC-P Image

GAPDH antibody detects GAPDH protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
GAPDH stained by GAPDH antibody (GTX100118) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GAPDH antibody used in IHC (Paraffin sections) (IHC-P). GTX100118

GTX100118 IHC-P Image

GAPDH antibody detects GAPDH protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
GAPDH stained by GAPDH antibody (GTX100118) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GAPDH antibody used in Western Blot (WB). GTX100118

GTX100118 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody (GTX100118) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GAPDH antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100118

GTX100118 ICC/IF Image

GAPDH antibody detects GAPDH protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GAPDH stained by GAPDH antibody (GTX100118) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Anti-GAPDH antibody used in Western Blot (WB). GTX100118

GTX100118 WB Image

Regulation of NED by REST in LNCaP cells.(A) The level of REST protein declines during IL-6 treatment. LNCaP cells were treated with 100 ng/ml IL-6 for 48 and 96 hours. The expression level of REST was analyzed by immunoblotting using anti-REST antibody. GAPDH was used as the loading control. (B) LNCaP-TR-shREST cells were treated with or without Dox for 48 hours. TCLs were analyzed by immunoblotting using anti-REST antibody. (C) LNCaP-TR-shREST cells were treated with Dox for 6 days. The promotion of neurite outgrowth by REST knockdown was assessed using brightfield microscopy images (40× magnification). (D) The neurite elongation was quantified using the average from 3–5 microscopic fields; bars, SD. (E) LNCaP-TR-shREST cells were treated as described in (C). TCLs were prepared and analyzed by immunoblotting using the antibodies as indicated. (F) LNCaP-TR-REST cells were treated with 1 µg/ml Dox in the absence (control) or presence of 100 ng/ml IL-6 for 4 days. Inhibition of IL-6-induced neurite outgrowth by REST overexpression was assessed using brightfield microscopy images (40× magnification). (G) TCLs were obtained from LNCaP-TR-REST cells treated as described in (F); these were then analyzed by immunoblotting using the indicated antibodies. (H) RT-qPCR analysis of total RNA isolated from LNCaP-TR-shREST cells treated as described in (C). The relative mRNA levels of REST, Atg5, beclin1 and LC3 were normalized against GAPDH. Values from three independent data points are reported as mean±S.D.

Anti-GAPDH antibody used in Western Blot (WB). GTX100118

GTX100118 WB Image

Regulation of NED by REST in LNCaP cells.(A) The level of REST protein declines during IL-6 treatment. LNCaP cells were treated with 100 ng/ml IL-6 for 48 and 96 hours. The expression level of REST was analyzed by immunoblotting using anti-REST antibody. GAPDH was used as the loading control. (B) LNCaP-TR-shREST cells were treated with or without Dox for 48 hours. TCLs were analyzed by immunoblotting using anti-REST antibody. (C) LNCaP-TR-shREST cells were treated with Dox for 6 days. The promotion of neurite outgrowth by REST knockdown was assessed using brightfield microscopy images (40× magnification). (D) The neurite elongation was quantified using the average from 3–5 microscopic fields; bars, SD. (E) LNCaP-TR-shREST cells were treated as described in (C). TCLs were prepared and analyzed by immunoblotting using the antibodies as indicated. (F) LNCaP-TR-REST cells were treated with 1 µg/ml Dox in the absence (control) or presence of 100 ng/ml IL-6 for 4 days. Inhibition of IL-6-induced neurite outgrowth by REST overexpression was assessed using brightfield microscopy images (40× magnification). (G) TCLs were obtained from LNCaP-TR-REST cells treated as described in (F); these were then analyzed by immunoblotting using the indicated antibodies. (H) RT-qPCR analysis of total RNA isolated from LNCaP-TR-shREST cells treated as described in (C). The relative mRNA levels of REST, Atg5, beclin1 and LC3 were normalized against GAPDH. Values from three independent data points are reported as mean±S.D.

Anti-GAPDH antibody used in Western Blot (WB). GTX100118

GTX100118 WB Image

Creation and characterization of Supt4h knockout mice.(A) Genomic organization of the mouse Supt4h locus (Top) and structure of the targeting vector (Middle). In the allele carrying the Supt4h deletion, a neo cassette specifying resistance to the antibiotic G418 in animal cells replaced the DNA fragment encompassing exon 2 to exon 5 of Supt4h via homologous recombination (Bottom). Positions of 5’ and 3’ flanking probes used in Southern blot analysis, and predicted sizes of restriction fragments detected by these probes are shown. Genomic DNA of C57BL6/129 mice (S+/+) and their Supt4h+/- (S+/-) littermates was subjected to Southern blot analysis using the 5’ and 3’ probes separately. (B) Supt4h mRNA levels were assessed by qRT-PCR using the brain tissue of Supt4h+/+ and Supt4h+/- mice. The abundance in Supt4h+/+ mice was set as 1, after normalization with U6 RNA. (C) SUPT4H protein level in the striatum and cortex of indicated mice was analyzed by immunohistochemistry (IHC) using antibody against SUPT4H. (D) Protein lysates collected from the cerebrum of indicated mice were analyzed by Western blot using anti-SUPT4H antibody. GAPDH served as loading control. Data are presented as the mean ± SEM (n = 3 in each group; *, p < 0.05; ***, p <0.001 by Student’s t-test). The mice were sacrificed at the age of 12 weeks for analyses.

Anti-GAPDH antibody used in Western Blot (WB). GTX100118

GTX100118 WB Image

IL-6 treatment inhibits mTOR via the activation of AMPK pathway.(A) LNCaP cells were treated for 48 hours with 2.5% CDT or 2.5% CDT plus 100 ng/ml IL-6. TCLs were prepared and immunoblotted using phospho-STAT3, phospho-Akt and phopho-ERK specific antibodies; immunoblotting to detect the non-phospho-counterparts of these proteins was used as the control. GAPDH was used as the loading control. (B) LNCaP cells were treated as described in (A) and immunoblotted using the antibodies as indicated and using GAPDH as the loading control.

Anti-GAPDH antibody used in Western Blot (WB). GTX100118

GTX100118 WB Image

Regulation of NED by REST in LNCaP cells.(A) The level of REST protein declines during IL-6 treatment. LNCaP cells were treated with 100 ng/ml IL-6 for 48 and 96 hours. The expression level of REST was analyzed by immunoblotting using anti-REST antibody. GAPDH was used as the loading control. (B) LNCaP-TR-shREST cells were treated with or without Dox for 48 hours. TCLs were analyzed by immunoblotting using anti-REST antibody. (C) LNCaP-TR-shREST cells were treated with Dox for 6 days. The promotion of neurite outgrowth by REST knockdown was assessed using brightfield microscopy images (40× magnification). (D) The neurite elongation was quantified using the average from 3–5 microscopic fields; bars, SD. (E) LNCaP-TR-shREST cells were treated as described in (C). TCLs were prepared and analyzed by immunoblotting using the antibodies as indicated. (F) LNCaP-TR-REST cells were treated with 1 µg/ml Dox in the absence (control) or presence of 100 ng/ml IL-6 for 4 days. Inhibition of IL-6-induced neurite outgrowth by REST overexpression was assessed using brightfield microscopy images (40× magnification). (G) TCLs were obtained from LNCaP-TR-REST cells treated as described in (F); these were then analyzed by immunoblotting using the indicated antibodies. (H) RT-qPCR analysis of total RNA isolated from LNCaP-TR-shREST cells treated as described in (C). The relative mRNA levels of REST, Atg5, beclin1 and LC3 were normalized against GAPDH. Values from three independent data points are reported as mean±S.D.

Anti-GAPDH antibody used in Western Blot (WB). GTX100118

GTX100118 WB Image

IL-6 treatment inhibits mTOR via the activation of AMPK pathway.(A) LNCaP cells were treated for 48 hours with 2.5% CDT or 2.5% CDT plus 100 ng/ml IL-6. TCLs were prepared and immunoblotted using phospho-STAT3, phospho-Akt and phopho-ERK specific antibodies; immunoblotting to detect the non-phospho-counterparts of these proteins was used as the control. GAPDH was used as the loading control. (B) LNCaP cells were treated as described in (A) and immunoblotted using the antibodies as indicated and using GAPDH as the loading control.

Anti-GAPDH antibody used in Western Blot (WB). GTX100118

GTX100118 WB Image

Various whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody (GTX100118) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW

GAPDH antibody Cat No. GTX100118

Rating ( Average 4.6 based on 16 users reviews)
Application
Western Blot(WB) ( Average 4.5 based on 15 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 13-Aug-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : SAS cells
Amount used : 29 μg
Blocking : BSA, 25°C, 1Hr
Primary Antibody : 1:5000, 4°C, 16Hr
Date : Anonymous submitted on 31-Jul-2017
Score :
Application Tested : WB
Sample Species : Hu
Sample : A549 cells
Amount used : 30 μg
Blocking : 5% skim milk, 25°C, 1Hr
Primary Antibody : 1:5000, 25°C, 2Hr
Date : Anonymous submitted on 29-Apr-2016
Score :
Application Tested : WB
Sample Species : Ms
Sample : Brown adipose tissue
Amount used : 32μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Date : Anonymous submitted on 07-Apr-2015
Score :
Application Tested : WB
Sample Species : Ms
Sample : skin tissue
Amount used : 20μg
Blocking : 5% non-fat milk, 25°C, 2Hr
Primary Antibody : 1:10000, 4°C, 16Hr
Date : Anonymous submitted on 27-Jan-2015
Score :
Application Tested : WB
Sample Species : Rat
Sample : A7R5 cells, H9C2 cells
Amount used : 40μg
Blocking : 3% BSA in TBST, 25°C, 1Hr
Primary Antibody : 1:10000, 4°C, 24Hr
Lane Description : Lane1: A7R5 cells Lane2: H9C2 cells
Date : Anonymous submitted on 09-Oct-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : U2OS cells
Amount used : 2μg
Blocking : 5% milk in 0.1%TBST, 25°C, 0.5Hr
Primary Antibody : 1:20000, 25°C, 1Hr
Date : Anonymous submitted on 07-Aug-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : human lung cancer cells
Amount used : 30μg
Blocking : 25°C, 2Hr
Primary Antibody : 1:10000, 4°C, 10Hr
Date : Anonymous submitted on 09-May-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : 293T cells
Amount used : 28μg
Blocking : 5% BSA in TBST, 4°C, 1Hr
Primary Antibody : 1:5000, 4°C, 14Hr
Date : Anonymous submitted on 05-Jun-2013
Score :
Application Tested : WB
Sample Species : Hu
Sample : A549 cells,HCC827 cells,H1975 cells,H358 cells
Amount used : 50μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:5000, 25°C, 1Hr
Date : Anonymous submitted on 16-Nov-2012
Score :
Application Tested : WB
Sample Species : Hu
Sample : HEK293T cells
Amount used : 30μg
Blocking : 1% BSA/TBST, 2Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Date : Anonymous submitted on 24-Aug-2012
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : HEK293 cells
Blocking : 1% BSA, 37°C, 0.5Hr
Fixation : 4% paraformaldehyde
Permeabilization /Antigen retrieval : 0.1% Triton X-100
Primary Antibody : 1:500, 37°C, 1Hr
Date : Anonymous submitted on 24-Aug-2012
Score :
Application Tested : WB
Sample : Drosophila eye disc tissue
Blocking : 5% milk in PBST, 25°C, 1Hr
Primary Antibody : 1:3000, 4°C, 16Hr
Date : Anonymous submitted on 30-Jan-2012
Score :
Application Tested : WB
Sample Species : Ms
Sample : Mouse cerebral endothelial tissue
Amount used : 30μg
Blocking : 5% skim milk in TBST, 4°C, overnightHr
Primary Antibody : 1:5000, 25°C, 2Hr
Date : Anonymous submitted on 16-Jan-2012
Score :
Application Tested : WB
Sample Species : Hu
Sample : SH-SY5Y cells
Amount used : 25μg
Blocking : 5% non-fat milk, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Date : Anonymous submitted on 16-Jan-2012
Score :
Application Tested : WB
Sample Species : Hu
Sample : A431 cells
Amount used : 30μg
Blocking : 5% BSA, 25°C, 1Hr
Primary Antibody : 1:60000, 4°C, 16Hr
Date : Anonymous submitted on 31-Oct-2011
Score :
Application Tested : WB
Sample Species : Hu
Sample : A549 cells
Amount used : 10μg
Blocking : 5% milk in TBST, 25°C, 2Hr
Primary Antibody : 1:1000, 25°C, 2Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Document
Unmodified.pdf
Package List Price ($)
$ 319
$ 169