GeneTex
United States (US)

GAPDH antibody

GTX100118_41577_20150327_IP_19011506_593.jpg
GTX100118_43006_20171101_ICC_IF_19011506_296.jpg
GTX100118_42116_20160630_WB_M_19011506_644.jpg
GTX100118_42977_20171027_WB_19011506_471.jpg
GTX100118_42158_20151217_WB_E. coli_19011720_774.jpg
GTX100118_42977_20170929_WB_Sensitivity_19011506_866.jpg
GTX100118_41577_WB_R_19011506_200.jpg
GTX100118_40352_IHC_19011506_717.jpg
GTX100118_43355_20190118_IHC-P_M_19022001_253.jpg
GTX100118_43005_20190118_IHC-P_R_19022001_659.jpg

Cat No. GTX100118

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse, Rat, Zebrafish, Yeast, Rabbit, Drosophila, Bovine, Dog, Hamster, Pig, Monkey, E. coli, Mosquito, Nematode, Pika, Fish, Bacteria
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:100000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2 , E.coli M15 , *SH-SY5Y , *A549 , *U2OS , *H9C2 , *A7R5 , *mouse skin tissue

Predict Reactivity

Sheep, Cat, Chicken, Guinea pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.69 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GAPDH. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Glyceraldehyde-3-Phosphate Dehydrogenase,G3Pd,Gapd,Hel-S-162Ep,Gapdh

Cellular Localization

Cytoplasm , perinuclear region , Membrane

Background

The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100118_41577_20150327_IP_19011506_593.jpg

GTX100118 IP Image

Immunoprecipitation of GAPDH protein from 293T whole cell extracts using 5 μg of GAPDH antibody (GTX100118).
Western blot analysis was performed using GAPDH antibody (GTX100118).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX100118_43006_20171101_ICC_IF_19011506_296.jpg

GTX100118 ICC/IF Image

GAPDH antibody detects GAPDH protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GAPDH stained by GAPDH antibody (GTX100118) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX100118_42116_20160630_WB_M_19011506_644.jpg

GTX100118 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody (GTX100118) diluted at 1:6000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100118_42977_20171027_WB_19011506_471.jpg

GTX100118 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody (GTX100118) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100118_42158_20151217_WB_E. coli_19011720_774.jpg

GTX100118 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody (GTX100118) diluted at 1:5000.

GTX100118_42977_20170929_WB_Sensitivity_19011506_866.jpg

GTX100118 WB Image

Various whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody (GTX100118) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100118_41577_WB_R_19011506_200.jpg

GTX100118 WB Image

GAPDH antibody detects GAPDH protein by western blot analysis.
A. 30 µg PC-12 whole cell lysate/extract
B. 30 µg Rat2 whole cell lysate/extract
10% SDS-PAGE
GAPDH antibody (GTX100118) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100118_40352_IHC_19011506_717.jpg

GTX100118 IHC-P Image

Immunohistochemical analysis of human salivary gland cancer, using GAPDH(GTX100118) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100118_43355_20190118_IHC-P_M_19022001_253.jpg

GTX100118 IHC-P Image

GAPDH antibody detects GAPDH protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
GAPDH stained by GAPDH antibody (GTX100118) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX100118_43005_20190118_IHC-P_R_19022001_659.jpg

GTX100118 IHC-P Image

GAPDH antibody detects GAPDH protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
GAPDH stained by GAPDH antibody (GTX100118) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
REVIEW

GAPDH antibody Cat No. GTX100118

Rating ( Average 4.6 based on 16 users reviews)
Application
Western Blot(WB) ( Average 4.5 based on 15 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 13-Aug-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : SAS cells
Amount used : 29 μg
Blocking : BSA, 25°C, 1Hr
Primary Antibody : 1:5000, 4°C, overnightHr
Date : Anonymous submitted on 31-Jul-2017
Score :
Application Tested : WB
Sample Species : Hu
Sample : A549 cells
Amount used : 30 μg
Blocking : 5% skim milk, 25°C, 1Hr
Primary Antibody : 1:5000, 25°C, 2Hr
Date : Anonymous submitted on 29-Apr-2016
Score :
Application Tested : WB
Sample Species : Ms
Sample : Brown adipose tissue
Amount used : 32μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Date : Anonymous submitted on 07-Apr-2015
Score :
Application Tested : WB
Sample Species : Ms
Sample : skin tissue
Amount used : 20μg
Blocking : 5% non-fat milk, 25°C, 2Hr
Primary Antibody : 1:10000, 4°C, overnightHr
Date : Anonymous submitted on 27-Jan-2015
Score :
Application Tested : WB
Sample Species : Rat
Sample : A7R5 cells, H9C2 cells
Amount used : 40μg
Blocking : 3% BSA in TBST, 25°C, 1Hr
Primary Antibody : 1:10000, 4°C, 24Hr
Lane Description : Lane1: A7R5 cells Lane2: H9C2 cells
Date : Anonymous submitted on 09-Oct-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : U2OS cells
Amount used : 2μg
Blocking : 5% milk in 0.1%TBST, 25°C, 0.5Hr
Primary Antibody : 1:20000, 25°C, 1Hr
Date : Anonymous submitted on 07-Aug-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : human lung cancer cells
Amount used : 30μg
Blocking : 25°C, 2Hr
Primary Antibody : 1:10000, 4°C, 10Hr
Date : Anonymous submitted on 09-May-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : 293T cells
Amount used : 28μg
Blocking : 5% BSA in TBST, 4°C, 1Hr
Primary Antibody : 1:5000, 4°C, 14Hr
Date : Anonymous submitted on 05-Jun-2013
Score :
Application Tested : WB
Sample Species : Hu
Sample : A549 cells,HCC827 cells,H1975 cells,H358 cells
Amount used : 50μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:5000, 25°C, 1Hr
Date : Anonymous submitted on 16-Nov-2012
Score :
Application Tested : WB
Sample Species : Hu
Sample : HEK293T cells
Amount used : 30μg
Blocking : 1% BSA/TBST, 2Hr
Primary Antibody : 1:1000, 4°C, overnightHr
Date : Anonymous submitted on 24-Aug-2012
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : HEK293 cells
Blocking : 1% BSA, 37°C, 0.5Hr
Fixation : 4% paraformaldehyde
Permeabilization /Antigen retrieval : 0.1% Triton X-100
Primary Antibody : 1:500, 37°C, 1Hr
Date : Anonymous submitted on 24-Aug-2012
Score :
Application Tested : WB
Sample : Drosophila eye disc tissue
Blocking : 5% milk in PBST, 25°C, 1Hr
Primary Antibody : 1:3000, 4°C, overnightHr
Date : Anonymous submitted on 30-Jan-2012
Score :
Application Tested : WB
Sample Species : Ms
Sample : Mouse cerebral endothelial tissue
Amount used : 30μg
Blocking : 5% skim milk in TBST, 4°C, overnightHr
Primary Antibody : 1:5000, 25°C, 2Hr
Date : Anonymous submitted on 16-Jan-2012
Score :
Application Tested : WB
Sample Species : Hu
Sample : SH-SY5Y cells
Amount used : 25μg
Blocking : 5% non-fat milk, 1Hr
Primary Antibody : 1:1000, 4°C, overnightHr
Date : Anonymous submitted on 16-Jan-2012
Score :
Application Tested : WB
Sample Species : Hu
Sample : A431 cells
Amount used : 30μg
Blocking : 5% BSA, 25°C, 1Hr
Primary Antibody : 1:60000, 4°C, overnightHr
Date : Anonymous submitted on 31-Oct-2011
Score :
Application Tested : WB
Sample Species : Hu
Sample : A549 cells
Amount used : 10μg
Blocking : 5% milk in TBST, 25°C, 2Hr
Primary Antibody : 1:1000, 25°C, 2Hr
Application Reference
#VALUE! et al. #VALUE! 2016; 3 (43416) #VALUE!
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Document
Unmodified.pdf
Package List Price ($)
$ 319
$ 169