GeneTex
United States (US)

GAPDHS antibody

GTX116000_40289_WB_M_18121410_694.jpg
GTX116000_40289_IHC_18121410_815.jpg
GTX116000_40289_IFA_18121410_872.jpg
GTX116000_WB_18121410_530.jpg
GTX116000_40289_20160324_WB_M_testis_18121410_175.jpg

Cat No. GTX116000

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

MCF-7 , NIH-3T3 , mouse testis

Predict Reactivity

Rat, Zebrafish, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.91mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GAPDHS. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

GAPD2 antibody, GAPDH-2 antibody, GAPDS antibody, HSD-35 antibody, GAPDHS antibody, spermatogenic glyceraldehyde-3-phosphate dehydrogenase antibody, glyceraldehyde-3-phosphate dehydrogenase, testis-specific antibody, spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase 2 antibody, glyceraldehyde-3-phosphate dehydrogenase, spermatogenic antibody

Cellular Localization

Cytoplasm

Background

This gene encodes a protein belonging to the glyceraldehyde-3-phosphate dehydrogenase family of enzymes that play an important role in carbohydrate metabolism. Like its somatic cell counterpart, this sperm-specific enzyme functions in a nicotinamide adenine dinucleotide-dependent manner to remove hydrogen and add phosphate to glyceraldehyde 3-phosphate to form 1,3-diphosphoglycerate. During spermiogenesis, this enzyme may play an important role in regulating the switch between different energy-producing pathways, and it is required for sperm motility and male fertility. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX116000_40289_WB_M_18121410_694.jpg

GTX116000 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
10% SDS PAGE
GTX116000 diluted at 1:5000

GTX116000_40289_IHC_18121410_815.jpg

GTX116000 IHC-P Image

GAPDHS antibody detects GAPDHS protein at cytosol on human by immunohistochemical analysis.
Sample: Paraffin-embedded gastric cancer.
GAPDHS antibody (GTX116000) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX116000_40289_IFA_18121410_872.jpg

GTX116000 ICC/IF Image

Immunofluorescence analysis of methanol-fixed MCF-7, using GAPDHS(GTX116000) antibody at 1:200 dilution.

GTX116000_WB_18121410_530.jpg

GTX116000 WB Image

Sample (30 ug of whole cell lysate)
A: MCF-7
10% SDS PAGE
GTX116000 diluted at 1:1000

GTX116000_40289_20160324_WB_M_testis_18121410_175.jpg

GTX116000 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAPDHS antibody (GTX116000) diluted at 1:500.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169