GeneTex
United States (US)

GAPDHS antibody

GTX116000_40289_WB_M_18121410_694.jpg
GTX116000_40289_IHC_18121410_815.jpg
GTX116000_40289_IFA_18121410_872.jpg
GTX116000_WB_18121410_530.jpg
GTX116000_40289_20160324_WB_M_testis_18121410_175.jpg

Cat No. GTX116000

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

MCF-7 , NIH-3T3 , mouse testis

Predict Reactivity

Rat, Zebrafish, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.91 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GAPDHS. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Glyceraldehyde-3-Phosphate Dehydrogenase, Spermatogenic,Gapd2,Gapdh-2,Gapds,Hel-S-278,Hsd-35,Gapdhs

Cellular Localization

Cytoplasm

Background

This gene encodes a protein belonging to the glyceraldehyde-3-phosphate dehydrogenase family of enzymes that play an important role in carbohydrate metabolism. Like its somatic cell counterpart, this sperm-specific enzyme functions in a nicotinamide adenine dinucleotide-dependent manner to remove hydrogen and add phosphate to glyceraldehyde 3-phosphate to form 1,3-diphosphoglycerate. During spermiogenesis, this enzyme may play an important role in regulating the switch between different energy-producing pathways, and it is required for sperm motility and male fertility. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX116000_40289_WB_M_18121410_694.jpg

GTX116000 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
10% SDS PAGE
GTX116000 diluted at 1:5000

GTX116000_40289_IHC_18121410_815.jpg

GTX116000 IHC-P Image

GAPDHS antibody detects GAPDHS protein at cytosol on human by immunohistochemical analysis.
Sample: Paraffin-embedded gastric cancer.
GAPDHS antibody (GTX116000) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX116000_40289_IFA_18121410_872.jpg

GTX116000 ICC/IF Image

Immunofluorescence analysis of methanol-fixed MCF-7, using GAPDHS(GTX116000) antibody at 1:200 dilution.

GTX116000_WB_18121410_530.jpg

GTX116000 WB Image

Sample (30 ug of whole cell lysate)
A: MCF-7
10% SDS PAGE
GTX116000 diluted at 1:1000

GTX116000_40289_20160324_WB_M_testis_18121410_175.jpg

GTX116000 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAPDHS antibody (GTX116000) diluted at 1:500.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169