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GARS antibody [N3C2], Internal

Anti-GARS antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX114421
Anti-GARS antibody [N3C2], Internal used in Western Blot (WB). GTX114421
Anti-GARS antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX114421

Cat. No. GTX114421

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all GARS products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

83 kDa. ( Note )

Positive Control

293T , A431 , Raji

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Chicken, Xenopus tropicalis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.37 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GARS. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10622906

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glycyl-tRNA synthetase , CMT2D , DSMAV , GlyRS , HMN5 , SMAD1

Background

This gene encodes glycyl-tRNA synthetase, one of the aminoacyl-tRNA synthetases that charge tRNAs with their cognate amino acids. The encoded enzyme is an (alpha)2 dimer which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GARS antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX114421

GTX114421 IHC-P Image

GARS antibody [N3C2], Internal detects GARS protein at cytosol on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
GARS antibody [N3C2], Internal (GTX114421) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GARS antibody [N3C2], Internal used in Western Blot (WB). GTX114421

GTX114421 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: A431 (GTX27909)
C: Raji
7.5% SDS PAGE
Glycyl-tRNA synthetase antibody
GTX114421 diluted at 1:1000

Anti-GARS antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX114421

GTX114421 IHC-P Image

GARS antibody [N3C2], Internal detects GARS protein at cytoplasm on human normal liver by immunohistochemical analysis.
Sample: Paraffin-embedded normal liver.
GARS antibody [N3C2], Internal (GTX114421) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169