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GART antibody [N1N2], N-term

Anti-GART antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX114422
Anti-GART antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114422
Anti-GART antibody [N1N2], N-term used in Western Blot (WB). GTX114422

Cat. No. GTX114422

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all GART products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

108 kDa. ( Note )

Positive Control

A431 , HeLa

Predict Reactivity

Mouse, Rat, Rabbit, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GART. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11171974

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase , AIRS , GARS , GARTF , PAIS , PGFT , PRGS

Background

The protein encoded by this gene is a trifunctional polypeptide. It has phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase activity which is required for de novo purine biosynthesis. This enzyme is highly conserved in vertebrates. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq]

Database

DATA IMAGES

Anti-GART antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX114422

GTX114422 IHC-P Image

GART antibody [N1N2], N-term detects GART protein at cytoplasm on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
GART antibody [N1N2], N-term (GTX114422) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GART antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114422

GTX114422 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using GART(GTX114422) antibody at 1:500 dilution.

Anti-GART antibody [N1N2], N-term used in Western Blot (WB). GTX114422

GTX114422 WB Image

Sample (30 ug of whole cell lysate)
A: A431
B: HeLa
7.5% SDS PAGE
GTX114422 diluted at 1:5000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169