GeneTex
United States (US)

GAS2L1 antibody

GTX106226_39911_IFA_19011506_316.jpg
GTX106226_39911_IHC_19011506_147.jpg
GTX106226_39911_WB_19011506_870.jpg
GTX106226_39911_WB_M_brain_19011506_569.jpg

Cat No. GTX106226

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

73 kDa. ( Note )

Positive Control

A431 , HeLa , A375 , mouse brain

Predict Reactivity

Rat, Bovine, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.8mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GAS2L1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

GAR22 antibody, MGC17243 antibody, GAS2L1 antibody, GAS2-like protein 1 antibody, growth arrest-specific protein 2-like 1 antibody, GAS2-related protein on chromosome 22 antibody, growth arrest-specific 2 like 1 antibody

Cellular Localization

Cytoplasm , cytoskeleton

Background

The protein encoded by this gene, a member of the GAS2 family, is similar in sequence to the mouse protein Gas2, an actin-associated protein expressed at high levels in growth-arrested cells. Expression of the mouse Gas2 gene is negatively regulated by serum and growth factors. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX106226_39911_IFA_19011506_316.jpg

GTX106226 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using GAS2L1(GTX106226) antibody at 1:500 dilution.

GTX106226_39911_IHC_19011506_147.jpg

GTX106226 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SCC4 xenograft, using GAS2L1(GTX106226) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX106226_39911_WB_19011506_870.jpg

GTX106226 WB Image

GAS2L1 antibody detects GAS2L1 protein by Western blot analysis.
A. 30 µg A431 whole cell lysate/extract
B. 30 µg HeLa whole cell lysate/extract
C. 30 µg A375 whole cell lysate/extract
10 % SDS-PAGE
GAS2L1 antibody (GTX106226) dilution: 1:1000

GTX106226_39911_WB_M_brain_19011506_569.jpg

GTX106226 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX106226 diluted at 1:500

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169