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GAS2L1 antibody

Anti-GAS2L1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106226
Anti-GAS2L1 antibody used in IHC (Paraffin sections) (IHC-P). GTX106226
Anti-GAS2L1 antibody used in Western Blot (WB). GTX106226
Anti-GAS2L1 antibody used in Western Blot (WB). GTX106226

Cat No. GTX106226

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

73 kDa. ( Note )

Positive Control

A431 , HeLa , A375 , mouse brain

Predict Reactivity

Rat, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.8 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GAS2L1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950340

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

growth arrest specific 2 like 1 , GAR22

Cellular Localization

Cytoplasm , cytoskeleton

Background

The protein encoded by this gene, a member of the GAS2 family, is similar in sequence to the mouse protein Gas2, an actin-associated protein expressed at high levels in growth-arrested cells. Expression of the mouse Gas2 gene is negatively regulated by serum and growth factors. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GAS2L1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106226

GTX106226 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using GAS2L1(GTX106226) antibody at 1:500 dilution.

Anti-GAS2L1 antibody used in IHC (Paraffin sections) (IHC-P). GTX106226

GTX106226 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SCC4 xenograft, using GAS2L1(GTX106226) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GAS2L1 antibody used in Western Blot (WB). GTX106226

GTX106226 WB Image

GAS2L1 antibody detects GAS2L1 protein by Western blot analysis.
A. 30 μg A431 whole cell lysate/extract
B. 30 μg HeLa whole cell lysate/extract
C. 30 μg A375 whole cell lysate/extract
10 % SDS-PAGE
GAS2L1 antibody (GTX106226) dilution: 1:1000

Anti-GAS2L1 antibody used in Western Blot (WB). GTX106226

GTX106226 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX106226 diluted at 1:500

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf