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GATA1 (phospho Ser142) antibody

Orthogonal
Anti-GATA1 (phospho Ser142) antibody used in Western Blot (WB). GTX50116
Anti-GATA1 (phospho Ser142) antibody used in IHC (Paraffin sections) (IHC-P). GTX50116

Cat. No. GTX50116

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399)
See all GATA1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of serine 142 (R-L-S(p)-P-D) derived from human GATA1.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

GATA binding protein 1 , ERYF1 , GATA-1 , GF-1 , GF1 , NF-E1 , NFE1 , XLANP , XLTDA , XLTT

Cellular Localization

Nucleus

Background

This gene encodes a protein which belongs to the GATA family of transcription factors. The protein plays an important role in erythroid development by regulating the switch of fetal hemoglobin to adult hemoglobin. Mutations in this gene have been associated with X-linked dyserythropoietic anemia and thrombocytopenia. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-GATA1 (phospho Ser142) antibody used in Western Blot (WB). GTX50116

GTX50116 WB Image

Orthogonal

WB analysis of extracts from HL-60 cells untreated or treated with PMA using GTX50116 GATA1 (phospho Ser142) antibody.

Anti-GATA1 (phospho Ser142) antibody used in IHC (Paraffin sections) (IHC-P). GTX50116

GTX50116 IHC-P Image

Orthogonal

IHC-P analysis of human breast carcinoma tissue using GTX50116 GATA1 (phospho Ser142) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399