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GATA2 antibody

Orthogonal
Anti-GATA2 antibody used in Western Blot (WB). GTX113441
Anti-GATA2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113441
Anti-GATA2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX113441

Cat. No. GTX113441

Reference 3
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all GATA2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Predict Reactivity

Bovine, Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.38 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GATA2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10617761

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

GATA binding protein 2 , DCML , IMD21 , MONOMAC , NFE1B

Background

This gene encodes a member of the GATA family of zinc-finger transcription factors that are named for the consensus nucleotide sequence they bind in the promoter regions of target genes. The encoded protein plays an essential role in regulating transcription of genes involved in the development and proliferation of hematopoietic and endocrine cell lineages. Alternative splicing results in multiple transcript variants.

Database

Research Area

DATA IMAGES

Anti-GATA2 antibody used in Western Blot (WB). GTX113441

GTX113441 WB Image

Orthogonal

293T whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GATA2 antibody (GTX113441) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GATA2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113441

GTX113441 ICC/IF Image

GATA2 antibody detects GATA2 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GATA2 stained by GATA2 antibody (GTX113441) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-GATA2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX113441

GTX113441 IHC-Fr Image

GATA2 antibody detects GATA2 protein by immunohistochemical analysis.
Sample: Frozen-sectioned rat E13.5 brain.
Green: GATA2 stained by GATA2 antibody (GTX113441) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

Application Reference

Cioccarelli C et al. Front Immunol 2021; 12 : 781352 IL1β Promotes TMPRSS2 Expression and SARS-CoV-2 Cell Entry Through the p38 MAPK-GATA2 Axis.
Fletcher PA et al. Front Endocrinol (Lausanne) 2019; 10 : 623 Cell Type- and Sex-Dependent Transcriptome Profiles of Rat Anterior Pituitary Cells.
Application : ICC/IF
Reactivity : Rat
Wu X et al. Cell 2017; (Epub) Intrinsic Immunity Shapes Viral Resistance of Stem Cells.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

GATA2 antibody Cat. No. GTX113441

Rating
( Average 5 based on 2 users reviews)
Application
Western Blot(WB)
( Average 5 based on 2 users reviews)
Anti-GATA2 antibody used in Western Blot (WB). GTX113441
Date : Anonymous submitted on 13-Jul-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : 22RV1 cells
Amount used : 20μg
Blocking : 5% milk, 24°C, 1Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Anti-GATA2 antibody used in Western Blot (WB). GTX113441
Date : Anonymous submitted on 05-Mar-2012
Score :
Application Tested : WB
Sample Species : Hu
Sample : H1299 cells
Amount used : 40μg
Blocking : 3% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 25°C, 1Hr
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169