GeneTex

  • Country / Location Selection

United States (US)

GATA3 antibody [1A12-1D9]

KO/KD Orthogonal Overexpression
Anti-GATA3 antibody [1A12-1D9] used in Immunoprecipitation (IP). GTX30600
Anti-GATA3 antibody [1A12-1D9] used in IHC (Paraffin sections) (IHC-P). GTX30600
Anti-GATA3 antibody [1A12-1D9] used in IHC (Paraffin sections) (IHC-P). GTX30600
Anti-GATA3 antibody [1A12-1D9] used in Western Blot (WB). GTX30600
Anti-GATA3 antibody [1A12-1D9] used in Western Blot (WB). GTX30600
Anti-GATA3 antibody [1A12-1D9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30600
Anti-GATA3 antibody [1A12-1D9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30600
Anti-GATA3 antibody [1A12-1D9] used in IHC (Paraffin sections) (IHC-P). GTX30600

Cat. No. GTX30600

Reference 3

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1A12-1D9

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all GATA3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 1:50-1:100
IHC-P 1:200-1:2000
IP 5μg
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Product Note

Knockdown/Knockout validation was supported by references (PMID:28805661)

PROPERTIES

Form

Liquid

Buffer

PBS, 1mg/ml BSA, 30% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified partial recombinant human GATA3 protein expressed in E. coli.

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_2887595

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

GATA binding protein 3 , HDR , HDRS

Cellular Localization

Nucleus

Background

This gene encodes a protein which belongs to the GATA family of transcription factors. The protein contains two GATA-type zinc fingers and is an important regulator of T-cell development and plays an important role in endothelial cell biology. Defects in this gene are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia. [provided by RefSeq, Nov 2009]

Database

Research Area

DATA IMAGES

Anti-GATA3 antibody [1A12-1D9] used in Immunoprecipitation (IP). GTX30600

GTX30600 IP Image

IP analysis of SH-SY5Y cell lysates using GTX30600 GATA3 antibody [1A12-1D9].
IP reaction : 5μl antibody / 250μg lysate
Dilution : 1:1000

Anti-GATA3 antibody [1A12-1D9] used in IHC (Paraffin sections) (IHC-P). GTX30600

GTX30600 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX30600 GATA3 antibody [1A12-1D9].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:1000

Anti-GATA3 antibody [1A12-1D9] used in IHC (Paraffin sections) (IHC-P). GTX30600

GTX30600 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX30600 GATA3 antibody [1A12-1D9].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:1000

Anti-GATA3 antibody [1A12-1D9] used in Western Blot (WB). GTX30600

GTX30600 WB Image

Orthogonal

WB analysis of 15 ug of SH-SY5Y whole cell lysate (WCL), SH-SY5Y nuclear extract (NE) using GTX30600 GATA3 antibody [1A12-1D9].
Dilution : 1:1000

Anti-GATA3 antibody [1A12-1D9] used in Western Blot (WB). GTX30600

GTX30600 WB Image

Overexpression

WB analysis of 15 ug of HEK293T cell lysate overexpressing GATA3-DDK (right lane) or empty vector control (left lane) using GTX30600 GATA3 antibody [1A12-1D9].
Dilution : 1:1000

Anti-GATA3 antibody [1A12-1D9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30600

GTX30600 ICC/IF Image

ICC/IF analysis of SH-SY5Y cells using GTX30600 GATA3 antibody [1A12-1D9]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:50 for at least 1 hour at room temperature

Anti-GATA3 antibody [1A12-1D9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30600

GTX30600 ICC/IF Image

ICC/IF analysis of MCF-7 cells using GTX30600 GATA3 antibody [1A12-1D9]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:50 for at least 1 hour at room temperature

Anti-GATA3 antibody [1A12-1D9] used in IHC (Paraffin sections) (IHC-P). GTX30600

GTX30600 IHC-P Image

IHC-P analysis of rat brain tissue using GTX30600 GATA3 antibody [1A12-1D9].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:200

REFERENCE

Application Reference

Nunomura S et al. J Allergy Clin Immunol 2021; The FADS mouse: A?novel mouse model of atopic keratoconjunctivitis.
Fattah FA et al. Hum Pathol 2018; (Epub) The Impact of the effectiveness of GATA3 as a prognostic factor in breast cancer.
Application : WB
Reactivity : Human
Li W et al. J Clin Invest 2017; (Epub) The FOXN3-NEAT1-SIN3A repressor complex promotes progression of hormonally responsive breast cancer.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

There are currently no reviews for GATA3 antibody [1A12-1D9] (GTX30600). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399