GeneTex
United States (US)

GATA3 antibody [1A12-1D9]

KO/KD Orthogonal Overexpression
GTX30600_1332_IHC-P_19011519_731.jpg
GTX30600_729_ICC-IF_19011519_371.jpg
GTX30600_1334_IHC-P_19011519_123.jpg
GTX30600_727_ICC-IF_19011519_433.jpg
GTX30600_1449_IP_19011519_529.jpg
GTX30600_2079_WB_19011519_669.jpg
GTX30600_1333_IHC-P_19011519_156.jpg
GTX30600_1843_WB_19011519_425.jpg

Cat No. GTX30600

Host Mouse
Clonality Monoclonal
Clone Name 1A12-1D9
Isotype IgG1
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000
ICC/IF 1:50-1:100
IHC-P 1:200-1:2000
IP 5μg
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Specificity/Sensitivity

Knockdown/Knockout validation was supported by references (PMID:28805661)
PROPERTIES

Form

Liquid

Buffer

PBS, 1mg/ml BSA, 30% glycerol, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified partial recombinant human GATA3 protein expressed in E. coli.

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Gata Binding Protein 3,Hdr,Hdrs,Gata3

Cellular Localization

Nucleus

Background

This gene encodes a protein which belongs to the GATA family of transcription factors. The protein contains two GATA-type zinc fingers and is an important regulator of T-cell development and plays an important role in endothelial cell biology. Defects in this gene are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia. [provided by RefSeq, Nov 2009]

Database

Research Area

DATA IMAGES
GTX30600_1332_IHC-P_19011519_731.jpg

GTX30600 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX30600 GATA3 antibody [1A12-1D9].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:1000

GTX30600_729_ICC-IF_19011519_371.jpg

GTX30600 ICC/IF Image

ICC/IF analysis of SH-SY5Y cells using GTX30600 GATA3 antibody [1A12-1D9]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:50 for at least 1 hour at room temperature

GTX30600_1334_IHC-P_19011519_123.jpg

GTX30600 IHC-P Image

IHC-P analysis of rat brain tissue using GTX30600 GATA3 antibody [1A12-1D9].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:200

GTX30600_727_ICC-IF_19011519_433.jpg

GTX30600 ICC/IF Image

ICC/IF analysis of MCF-7 cells using GTX30600 GATA3 antibody [1A12-1D9]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:50 for at least 1 hour at room temperature

GTX30600_1449_IP_19011519_529.jpg

GTX30600 IP Image

IP analysis of SH-SY5Y cell lysates using GTX30600 GATA3 antibody [1A12-1D9].
IP reaction : 5μl antibody / 250μg lysate
Dilution : 1:1000

GTX30600_2079_WB_19011519_669.jpg

GTX30600 WB Image

Overexpression

WB analysis of 15 ug of HEK293T cell lysate overexpressing GATA3-DDK (right lane) or empty vector control (left lane) using GTX30600 GATA3 antibody [1A12-1D9].
Dilution : 1:1000

GTX30600_1333_IHC-P_19011519_156.jpg

GTX30600 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX30600 GATA3 antibody [1A12-1D9].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:1000

GTX30600_1843_WB_19011519_425.jpg

GTX30600 WB Image

Orthogonal

WB analysis of 15 ug of SH-SY5Y whole cell lysate (WCL), SH-SY5Y nuclear extract (NE) using GTX30600 GATA3 antibody [1A12-1D9].
Dilution : 1:1000

REFERENCE
Package List Price ($)
$ 319