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GATA3 antibody [GT1217]

Recombinant antibody Orthogonal
Anti-GATA3 antibody [GT1217] used in Western Blot (WB). GTX01198
Anti-GATA3 antibody [GT1217] used in Western Blot (WB). GTX01198

Cat. No. GTX01198

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 GT1217

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399)
See all GATA3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Observed MW

50-55 kDa. The observed M.W. is based on the following publication. PMID: 25753145

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human GATA3.

Purification


Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

GATA binding protein 3 , GATA3 , HDR , HDRS

Cellular Localization

Nucleus

Background

This gene encodes a protein which belongs to the GATA family of transcription factors. The protein contains two GATA-type zinc fingers and is an important regulator of T-cell development and plays an important role in endothelial cell biology. Defects in this gene are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia. [provided by RefSeq, Nov 2009]

Database

Research Area

DATA IMAGES

Anti-GATA3 antibody [GT1217] used in Western Blot (WB). GTX01198

GTX01198 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GATA3 antibody [GT1217] (GTX01198) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
The observed expression level is based on the publication: PMID: 25753145

Anti-GATA3 antibody [GT1217] used in Western Blot (WB). GTX01198

GTX01198 WB Image

WB analysis of various samples using GTX01198 GATA3 antibody [GT1217].
Dilution : 1:1000
Loading : 25μg

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399