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GATA3 antibody, N-term

Anti-GATA3 antibody, N-term used in Western Blot (WB). GTX89886
Anti-GATA3 antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX89886

Cat. No. GTX89886

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all GATA3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.3-1μg/ml
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89886-PEP).

Product Note

This antibody is expected to recognize both reported isoforms (NP_001002295.1 and NP_002042.1).

Predict Reactivity

Rat, Bovine, Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence EVTADQPRWVSHH-C, from the N Terminus of the protein sequence according to NP_001002295.1; NP_002042.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

GATA binding protein 3 , HDR , HDRS

Cellular Localization

Nucleus

Background

This gene encodes a protein which belongs to the GATA family of transcription factors. The protein contains two GATA-type zinc fingers and is an important regulator of T-cell development and plays an important role in endothelial cell biology. Defects in this gene are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia. [provided by RefSeq, Nov 2009]

Database

Research Area

DATA IMAGES

Anti-GATA3 antibody, N-term used in Western Blot (WB). GTX89886

GTX89886 WB Image

WB analysis of human tonsil (A) and mouse kidney (B) lysate using GTX89886 GATA3 antibody, N-term.
Dilution : 0.5μg/ml
Loading : 35μg protein in RIPA buffer

Anti-GATA3 antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX89886

GTX89886 IHC-P Image

IHC-P analysis of human tonsil using GTX89886 GATA3 antibody, N-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 3μg/ml
A) staining of cells in the germinal centre, B) staining of cells in the interfollicular area.

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399