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GATA3 antibody

Orthogonal Overexpression
Anti-GATA3 antibody used in Western Blot (WB). GTX54527
Anti-GATA3 antibody used in Western Blot (WB). GTX54527
Anti-GATA3 antibody used in IHC (Paraffin sections) (IHC-P). GTX54527
Anti-GATA3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54527
Anti-GATA3 antibody used in Western Blot (WB). GTX54527
Anti-GATA3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54527

Cat. No. GTX54527

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human
Package
100 μg ($399)
See all GATA3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1500
ICC/IF 1-2 μg/ml
IHC-P 1:50-1:500
FACS 3-5 μg/10⁶ cells
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1mg/ml BSA, 30% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Purified partial recombinant GATA3 protein expressed in E. coli

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

GATA binding protein 3 , HDR , HDRS

Cellular Localization

Nucleus

Background

This gene encodes a protein which belongs to the GATA family of transcription factors. The protein contains two GATA-type zinc fingers and is an important regulator of T-cell development and plays an important role in endothelial cell biology. Defects in this gene are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia. [provided by RefSeq, Nov 2009]

Database

Research Area

DATA IMAGES

Anti-GATA3 antibody used in Western Blot (WB). GTX54527

GTX54527 WB Image

WB analysis of whole cell extracts (20 ug lysate) of SH-SY5Y (Lane 1), Jurkat (Lane 2) and K-562 (Lane 3) using GTX54527 GATA3 antibody.
Dilution : 1:500-1:1500

Anti-GATA3 antibody used in Western Blot (WB). GTX54527

GTX54527 WB Image

Overexpression

WB analysis of 20 ug of HEK293T cell lysate overexpressing GATA3-DDK (right lane) or empty vector control (left lane) using GTX54527 GATA3 antibody.
Dilution : 1:1000

Anti-GATA3 antibody used in IHC (Paraffin sections) (IHC-P). GTX54527

GTX54527 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX54527 GATA3 antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:200

Anti-GATA3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54527

GTX54527 ICC/IF Image

ICC/IF analysis of MCF7 (left panel) and SH-SY5Y cells (right panel) using GTX54527 GATA3 antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:50 for at least 1 hour at room temperature

Anti-GATA3 antibody used in Western Blot (WB). GTX54527

GTX54527 WB Image

Orthogonal

WB analysis of 15 ug of SH-SY5Y whole cell lysate (WCL), SH-SY5Y nuclear extract (NE) using GTX54527 GATA3 antibody.
Dilution : 1:1000

Anti-GATA3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54527

GTX54527 ICC/IF Image

ICC/IF analysis of MCF-7 cells using GTX54527 GATA3 antibody. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton„ X-100 for 10 minutes
Dilution : 1 μg/ml in 0.1% BSA incubated for 3 hours at room temperature

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399