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GATA4 antibody [1H8]

Overexpression
Anti-GATA4 antibody [1H8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84461
Anti-GATA4 antibody [1H8] used in Western Blot (WB). GTX84461

Cat. No. GTX84461

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1H8

Isotype

 IgG2b

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($509)
See all GATA4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment expressed in E.coli corresponding to amino acids 296-442of human GATA4

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

GATA binding protein 4 , ASD2 , TACHD , TOF , VSD1

Cellular Localization

Nucleus

Background

Transcriptional activator. Binds to the consensus sequence 5'-AGATAG-3'. Acts as a transcriptional activator of ANF in cooperation with NKX2-5.

Database

Research Area

DATA IMAGES

Anti-GATA4 antibody [1H8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84461

GTX84461 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with GATA4 plasmid using GTX84461 GATA4 antibody [1H8].

Anti-GATA4 antibody [1H8] used in Western Blot (WB). GTX84461

GTX84461 WB Image

Overexpression

WB analysis of HEK293T cells transfected with GATA4 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84461 GATA4 antibody [1H8].
Loading : 5 ug per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 509