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GATA4 antibody

Anti-GATA4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113194
Anti-GATA4 antibody used in Western Blot (WB). GTX113194
Anti-GATA4 antibody used in IHC (Paraffin sections) (IHC-P). GTX113194

Cat No. GTX113194

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

HepG2 nuclear extract , *mouse heart

Predict Reactivity

Rat, Bovine, Dog, Chicken, Pig, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.3 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GATA4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

GATA binding protein 4,ASD2,TACHD,TOF,VSD1

Cellular Localization

Nucleus

Background

This gene encodes a member of the GATA family of zinc-finger transcription factors. Members of this family recognize the GATA motif which is present in the promoters of many genes. This protein is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function. Mutations in this gene have been associated with cardiac septal defects. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-GATA4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113194

GTX113194 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using GATA4(GTX113194) antibody at 1:500 dilution.

Anti-GATA4 antibody used in Western Blot (WB). GTX113194

GTX113194 WB Image

HepG2 whole cell and nuclear extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with GATA4 antibody (GTX113194) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GATA4 antibody used in IHC (Paraffin sections) (IHC-P). GTX113194

GTX113194 IHC-P Image

GATA4 antibody detects GATA4 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
GATA4 stained by GATA4 antibody (GTX113194) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
REVIEW

GATA4 antibody Cat No. GTX113194

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 15-Apr-2014
Score :
Application Tested : WB
Sample Species : Ms
Sample : mouse heart tissue
Amount used : 8μg
Blocking : 3% milk in PBST, 23°C, 1Hr
Primary Antibody : 1:500, 4°C, 20Hr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169