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GATA4 antibody

Orthogonal Overexpression
Anti-GATA4 antibody used in Western Blot (WB). GTX54530
Anti-GATA4 antibody used in IHC (Paraffin sections) (IHC-P). GTX54530
Anti-GATA4 antibody used in IHC (Paraffin sections) (IHC-P). GTX54530
Anti-GATA4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54530
Anti-GATA4 antibody used in Flow cytometry (FACS). GTX54530
Anti-GATA4 antibody used in IHC (Paraffin sections) (IHC-P). GTX54530
Anti-GATA4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54530
Anti-GATA4 antibody used in Flow cytometry (FACS). GTX54530
Anti-GATA4 antibody used in Western Blot (WB). GTX54530
Anti-GATA4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54530
Anti-GATA4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54530
Anti-GATA4 antibody used in Flow cytometry (FACS). GTX54530
Anti-GATA4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54530

Cat. No. GTX54530

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all GATA4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
ICC/IF 1:50-1:200
IHC-P 1:100-1:1000
FACS 3-5 μg/10⁶ cells
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1mg/ml BSA, 30% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Pooled peptides: RKEGIQTRKRKPKNLNKSK and CKQDSWNSLVLADSHGDIITA

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

GATA binding protein 4 , ASD2 , TACHD , TOF , VSD1

Cellular Localization

Nucleus

Background

This gene encodes a member of the GATA family of zinc-finger transcription factors. Members of this family recognize the GATA motif which is present in the promoters of many genes. This protein is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function, and is necessary for normal testicular development. Mutations in this gene have been associated with cardiac septal defects. Additionally, alterations in gene expression have been associated with several cancer types. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]

Database

Research Area

DATA IMAGES

Anti-GATA4 antibody used in Western Blot (WB). GTX54530

GTX54530 WB Image

WB analysis of whole cell extracts (20 μg lysate) of SH-SY5Y (Lane 1) and Hep G2 (Lane 2) using GTX54530 GATA4 antibody.
Dilution : 1:500-1:2000

Anti-GATA4 antibody used in IHC (Paraffin sections) (IHC-P). GTX54530

GTX54530 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX54530 GATA4 antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0) microwaved for 8-15 min
Dilution : 1:200

Anti-GATA4 antibody used in IHC (Paraffin sections) (IHC-P). GTX54530

GTX54530 IHC-P Image

IHC-P analysis of mouse pancreas tissue using GTX54530 GATA4 antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0) microwaved for 8-15 min
Dilution : 1:500

Anti-GATA4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54530

GTX54530 ICC/IF Image

ICC/IF analysis of NIH-3T3 cells using GTX54530 GATA4 antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 and incubated overnight at 4ºC

Anti-GATA4 antibody used in Flow cytometry (FACS). GTX54530

GTX54530 FACS Image

FACS analysis of Hela cells using GTX54530 GATA4 antibody compared to an isotype control (blue).
Dilution : 2 ug/test for 60 min at room temperature
Fixation : 2% paraformaldehyde
Permeabilization : 90% methanol and incubated for 10 minutes at room temperature

Anti-GATA4 antibody used in IHC (Paraffin sections) (IHC-P). GTX54530

GTX54530 IHC-P Image

IHC-P analysis of human small intestine using GTX54530 GATA4 antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0) microwaved for 8-15 min
Dilution : 1:200

Anti-GATA4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54530

GTX54530 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX54530 GATA4 antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:200 incubated overnight at 4 ºC

Anti-GATA4 antibody used in Flow cytometry (FACS). GTX54530

GTX54530 FACS Image

FACS analysis of NIH/3T3 cells using GTX54530 GATA4 antibody compared to an isotype control (blue).
Dilution : 2 ug/test for 60 min at room temperature
Fixation : 2% paraformaldehyde
Permeabilization : 90% methanol and incubated for 10 minutes at room temperature

Anti-GATA4 antibody used in Western Blot (WB). GTX54530

GTX54530 WB Image

Orthogonal Overexpression

WB analysis of 40 ug of HeLa nuclear fraction (NF) (Lane 1), HEK293T control (Lane 2), and HEK293T GATA4 over-expression lysate (Lane 3) using GTX54530 GATA4 antibody.
Dilution : 1:1000

Anti-GATA4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54530

GTX54530 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX54530 GATA4 antibody.
Green : Primary antibody
Blue : cell nucleus
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:50 for 1 hour at room temperature

Anti-GATA4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54530

GTX54530 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX54530 GATA4 antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:200 incubated overnight at 4 ºC

Anti-GATA4 antibody used in Flow cytometry (FACS). GTX54530

GTX54530 FACS Image

FACS analysis of HepG2 cells using GTX54530 GATA4 antibody compared to an isotype control (blue).
Dilution : 2 ug/test for 60 min at room temperature
Fixation : 2% paraformaldehyde
Permeabilization : 90% methanol and incubated for 10 minutes at room temperature

Anti-GATA4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54530

GTX54530 ICC/IF Image

ICC/IF analysis of permeabilized embryoid body endoderm generated from human episomal iPSC using GTX54530 GATA4 antibody.
Green : Primary antibody
Blue : cell nucleus
Dilution : 1:100 in 3% BSA/PBS blocking buffer overnight at 4ºC

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399