WB analysis of cardiomyocytes overexpressing GATA-4 mutant(S105A) (Lane 1), wild type GATA-4 (Lane 2) stimulated with PE, (Lane 3) phosphopeptide using GTX25245 GATA4 (phospho Ser105) antibody prior incubated with non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 3) a generic phosphothreonine-containing peptide (Lane 4), or the phosphopeptide immunogen (Lane 5).Mutant analysis and peptide competition demonstrate antibody phospho-site specificity

IHC-P analysis of mouse lymph node using GTX25245 GATA4 (phospho Ser105) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20