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GATA4 (phospho Ser105) antibody

Orthogonal
Anti-GATA4 (phospho Ser105) antibody used in IHC (Paraffin sections) (IHC-P). GTX25245
Anti-GATA4 (phospho Ser105) antibody used in Western Blot (WB). GTX25245

Cat. No. GTX25245

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
50 μl ($399)
See all GATA4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500
IHC-P 1:10-1:50
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 1mg/ml BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human GATA-4 that contains serine 105.

Purification

Purified IgG

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

GATA binding protein 4 , ASD2 , TACHD , TOF , VSD1

Cellular Localization

Nucleus

Background

This gene encodes a member of the GATA family of zinc-finger transcription factors. Members of this family recognize the GATA motif which is present in the promoters of many genes. This protein is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function, and is necessary for normal testicular development. Mutations in this gene have been associated with cardiac septal defects. Additionally, alterations in gene expression have been associated with several cancer types. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]

Database

Research Area

DATA IMAGES

Anti-GATA4 (phospho Ser105) antibody used in IHC (Paraffin sections) (IHC-P). GTX25245

GTX25245 IHC-P Image

IHC-P analysis of mouse lymph node using GTX25245 GATA4 (phospho Ser105) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20

Anti-GATA4 (phospho Ser105) antibody used in Western Blot (WB). GTX25245

GTX25245 WB Image

Orthogonal

WB analysis of cardiomyocytes overexpressing GATA-4 mutant(S105A) (Lane 1), wild type GATA-4 (Lane 2) stimulated with PE, (Lane 3) phosphopeptide using GTX25245 GATA4 (phospho Ser105) antibody prior incubated with non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 3) a generic phosphothreonine-containing peptide (Lane 4), or the phosphopeptide immunogen (Lane 5).Mutant analysis and peptide competition demonstrate antibody phospho-site specificity

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399