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GATA6 antibody

Anti-GATA6 antibody used in IHC (Paraffin sections) (IHC-P). GTX54528
Anti-GATA6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54528
Anti-GATA6 antibody used in Western Blot (WB). GTX54528

Cat. No. GTX54528

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all GATA6 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 1:200
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1mg/ml BSA, 30% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Synthetic peptide sequence KYSGQDGLYIGVSLASPAE and KRFGAAGADASDSRAFPARE

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

GATA binding protein 6

Cellular Localization

Nucleus

Background

This gene is a member of a small family of zinc finger transcription factors that play an important role in the regulation of cellular differentiation and organogenesis during vertebrate development. This gene is expressed during early embryogenesis and localizes to endo- and mesodermally derived cells during later embryogenesis and thereby plays an important role in gut, lung, and heart development. Mutations in this gene are associated with several congenital defects. [provided by RefSeq, Mar 2012]

Database

Research Area

DATA IMAGES

Anti-GATA6 antibody used in IHC (Paraffin sections) (IHC-P). GTX54528

GTX54528 IHC-P Image

IHC-P analysis of human stomach tissue using GTX54528 GATA6 antibody.
Antigen retrieval : 10mM sodium citrate followed by microwave treatment for 8-15 minutes.
Dilution : 1:100

Anti-GATA6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54528

GTX54528 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX54528 GATA6 antibody.
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:200 for at least 1 hour at room temperature

Anti-GATA6 antibody used in Western Blot (WB). GTX54528

GTX54528 WB Image

WB analysis of 25 ug of various whole cell lysate using GTX54528 GATA6 antibody.
Dilution : 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399