GeneTex
United States (US)

GBA antibody [C1C3]

Orthogonal
GTX101267_43222_20180518_WB_Fraction_19011519_473.jpg
GTX101267_WB_19011506_578.jpg
GTX101267_39904_IHC_19011506_771.jpg
GTX101267_39904_IFA_19011506_651.jpg

Cat No. GTX101267

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji

Predict Reactivity

Mouse, Rat, Bovine, Pig, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.84mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human GBA. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

GBA1 antibody, GCB antibody, GLUC antibody, GBA antibody, imiglucerase antibody, lysosomal glucocerebrosidase antibody, glucosylceramidase antibody, acid beta-glucosidase antibody, beta-glucocerebrosidase antibody, D-glucosyl-N-acylsphingosine glucohydrolase antibody, alglucerase antibody, "glucosidase, beta, acid antibody"

Cellular Localization

Lysosome membrane; Peripheral membrane protein; Lumenal side

Background

This gene encodes a lysosomal membrane protein that cleaves the beta-glucosidic linkage of glycosylceramide, an intermediate in glycolipid metabolism. Mutations in this gene cause Gaucher disease, a lysosomal storage disease characterized by an accumulation of glucocerebrosides. A related pseudogene is approximately 12 kb downstream of this gene on chromosome 1. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101267_43222_20180518_WB_Fraction_19011519_473.jpg

GTX101267 WB Image

Orthogonal

MCF-7 whole cell and membrane extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with GBA antibody [C1C3] (GTX101267) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101267_WB_19011506_578.jpg

GTX101267 WB Image

Sample(30 µg of whole cell lysate)
A:Hep G2(GTX27900)
7.5% SDS PAGE
GTX101267 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101267_39904_IHC_19011506_771.jpg

GTX101267 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human lung cancer, using GBA(GTX101267) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101267_39904_IFA_19011506_651.jpg

GTX101267 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using GBA(GTX101267) antibody at 1:100 dilution.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169