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GBA antibody [C1C3]

KO/KD Orthogonal
Anti-GBA antibody [C1C3] used in Western Blot (WB). GTX101267
Anti-GBA antibody [C1C3] used in Western Blot (WB). GTX101267
Anti-GBA antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX101267
Anti-GBA antibody [C1C3] used in Western Blot (WB). GTX101267
Anti-GBA antibody [C1C3] used in Western Blot (WB). GTX101267
Anti-GBA antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101267

Cat. No. GTX101267

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all GBA products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Positive Control

HepG2 , Raji

Predict Reactivity

Mouse, Rat, Bovine, Pig, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.32 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human GBA. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950346

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glucosylceramidase beta , GBA1 , GCB , GLUC

Cellular Localization

Lysosome membrane; Peripheral membrane protein; Lumenal side

Background

This gene encodes a lysosomal membrane protein that cleaves the beta-glucosidic linkage of glycosylceramide, an intermediate in glycolipid metabolism. Mutations in this gene cause Gaucher disease, a lysosomal storage disease characterized by an accumulation of glucocerebrosides. A related pseudogene is approximately 12 kb downstream of this gene on chromosome 1. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GBA antibody [C1C3] used in Western Blot (WB). GTX101267

GTX101267 WB Image

KO/KD

Wild-type (WT) and GBA knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GBA antibody [C1C3] (GTX101267) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GBA antibody [C1C3] used in Western Blot (WB). GTX101267

GTX101267 WB Image

Orthogonal

MCF-7 whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GBA antibody [C1C3] (GTX101267) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GBA antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX101267

GTX101267 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human lung cancer, using GBA(GTX101267) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GBA antibody [C1C3] used in Western Blot (WB). GTX101267

GTX101267 WB Image

Sample(30 μg of whole cell lysate)
A:Hep G2(GTX27900)
7.5% SDS PAGE
GTX101267 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GBA antibody [C1C3] used in Western Blot (WB). GTX101267

GTX101267 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GBA antibody [C1C3] (GTX101267) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-GBA antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101267

GTX101267 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using GBA(GTX101267) antibody at 1:100 dilution.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169