IP analysis of DDDDK tagged GBE1 overexpressed HEK293T lysate using GTX84456 GBE1 antibody [3B4].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody.
IP reaction : 2μg antibody / 500μl cell lysate

IHC-P analysis of human kidney tissue using GTX84456 GBE1 antibody [3B4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

IHC-P analysis of human prostate carcinoma tissue using GTX84456 GBE1 antibody [3B4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

WB analysis of COS-7 and MDCK cell lysate using GTX84456 GBE1 antibody [3B4].
Loading : 10 ug per lane
Dilution : 1:200

WB analysis of HEK293T cells transfected with GBE1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84456 GBE1 antibody [3B4].
Loading : 5 ug per lane

IHC-P analysis of human pancreas tissue using GTX84456 GBE1 antibody [3B4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

IHC-P analysis of human liver tissue using GTX84456 GBE1 antibody [3B4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

IHC-P analysis of human liver carcinoma tissue using GTX84456 GBE1 antibody [3B4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.