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GBF1 antibody [N1], N-term

Anti-GBF1 antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX102644
Anti-GBF1 antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102644
Anti-GBF1 antibody [N1], N-term used in Western Blot (WB). GTX102644

Cat. No. GTX102644

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all GBF1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

206 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human GBF1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10617495

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

golgi brefeldin A resistant guanine nucleotide exchange factor 1 , ARF1GEF

Background

This gene encodes a member of the Sec7 domain family. The encoded protein is a guanine nucleotide exchange factor that regulates the recruitment of proteins to membranes by mediating GDP to GTP exchange. The encoded protein is localized to the Golgi apparatus and plays a role in vesicular trafficking by activating ADP ribosylation factor 1. The encoded protein has also been identified as an important host factor for viral replication. Multiple transcript variants have been observed for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GBF1 antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX102644

GTX102644 IHC-P Image

GBF1 antibody [N1], N-term detects GBF1 protein at cytoplasm on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
GBF1 antibody [N1], N-term (GTX102644) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GBF1 antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102644

GTX102644 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using GBF1(GTX102644) antibody at 1:200 dilution.

Anti-GBF1 antibody [N1], N-term used in Western Blot (WB). GTX102644

GTX102644 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
5% SDS PAGE
GBF1 antibody
GTX102644 diluted at 1:1000

REFERENCE

Application Reference

Carpp LN et al. Mol Cell Proteomics 2014; 13 (11) : 2836-54 Quantitative proteomic analysis of host-virus interactions reveals a role for Golgi brefeldin A resistance factor 1 (GBF1) in dengue infection.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169