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GBP3 antibody [N1C1]

Anti-GBP3 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112554
Anti-GBP3 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX112554
Anti-GBP3 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX112554
Anti-GBP3 antibody [N1C1] used in Western Blot (WB). GTX112554

Cat. No. GTX112554

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all GBP3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Positive Control

HeLa

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.81 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GBP3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037128

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

guanylate binding protein 3

Cellular Localization

Membrane; Multi-pass membrane protein

Background

This gene encodes a member of the guanylate-binding protein (GBP) family. GBPs specifically bind guanine nucleotides (GMP, GDP, and GTP) and contain two of the three consensus motifs found in typical GTP-binding proteins. The encoded protein interacts with a member of the germinal center kinase family. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GBP3 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112554

GTX112554 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using Guanylate binding protein 3(GTX112554) antibody at 1:200 dilution.

Anti-GBP3 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX112554

GTX112554 IHC-P Image

GBP3 antibody [N1C1] detects GBP3 protein at cytoplasm in human colon by immunohistochemical analysis.
Sample: Paraffin-embedded human colon.
GBP3 antibody [N1C1] (GTX112554) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GBP3 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX112554

GTX112554 IHC-P Image

GBP3 antibody [N1C1] detects GBP3 protein at cytoplasm in human endometrium by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrium.
GBP3 antibody [N1C1] (GTX112554) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GBP3 antibody [N1C1] used in Western Blot (WB). GTX112554

GTX112554 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
7.5% SDS PAGE
GTX112554 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169