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GBX2 antibody

Orthogonal
Anti-GBX2 antibody used in Western Blot (WB). GTX129494
Anti-GBX2 antibody used in Western Blot (WB). GTX129494
Anti-GBX2 antibody used in Western Blot (WB). GTX129494
Anti-GBX2 antibody used in Western Blot (WB). GTX129494
Anti-GBX2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129494
Anti-GBX2 antibody used in Western Blot (WB). GTX129494
Anti-GBX2 antibody used in Western Blot (WB). GTX129494

Cat. No. GTX129494

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all GBX2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Positive Control

IMR32 nuclear

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.41 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GBX2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886017

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

gastrulation brain homeobox 2

Cellular Localization

Nucleus

Background

May act as a transcription factor for cell pluripotency and differentiation in the embryo.

Database

Research Area

DATA IMAGES

Anti-GBX2 antibody used in Western Blot (WB). GTX129494

GTX129494 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GBX2 antibody (GTX129494) diluted at 1:1000.

Anti-GBX2 antibody used in Western Blot (WB). GTX129494

GTX129494 WB Image

GBX2 antibody detects GBX2 protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GBX2 antibody (GTX129494) diluted by 1:1000.

Anti-GBX2 antibody used in Western Blot (WB). GTX129494

GTX129494 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GBX2 antibody (GTX129494) diluted at 1:1000.

Anti-GBX2 antibody used in Western Blot (WB). GTX129494

GTX129494 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GBX2 antibody (GTX129494) diluted at 1:1000.

Anti-GBX2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129494

GTX129494 ICC/IF Image

GBX2 antibody detects GBX2 protein at nucleus by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GBX2 protein stained by GBX2 antibody (GTX129494) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:50.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-GBX2 antibody used in Western Blot (WB). GTX129494

GTX129494 WB Image

Orthogonal

GBX2 antibody detects GBX2 protein by western blot analysis. IMR32 whole cell extracts and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GBX2 antibody (GTX129494) diluted by 1:1000.

Anti-GBX2 antibody used in Western Blot (WB). GTX129494

GTX129494 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GBX2 antibody (GTX129494) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169