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GBX2 antibody

Anti-GBX2 antibody used in Western Blot (WB). GTX129494
Anti-GBX2 antibody used in Western Blot (WB). GTX129494
Anti-GBX2 antibody used in Western Blot (WB). GTX129494
Anti-GBX2 antibody used in Western Blot (WB). GTX129494
Anti-GBX2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129494
Anti-GBX2 antibody used in Western Blot (WB). GTX129494

Cat No. GTX129494

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Positive Control

U87-MG , SK-N-SH , IMR32 , IMR32 nuclear , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , mouse fetal brain , rat brain

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GBX2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

gastrulation brain homeobox 2

Cellular Localization

Nucleus

Background

May act as a transcription factor for cell pluripotency and differentiation in the embryo.

Database

Research Area

DATA IMAGES

Anti-GBX2 antibody used in Western Blot (WB). GTX129494

GTX129494 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GBX2 antibody (GTX129494) diluted at 1:1000.

Anti-GBX2 antibody used in Western Blot (WB). GTX129494

GTX129494 WB Image

GBX2 antibody detects GBX2 protein by western blot analysis. IMR32 whole cell extracts and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GBX2 antibody (GTX129494) diluted by 1:1000.

Anti-GBX2 antibody used in Western Blot (WB). GTX129494

GTX129494 WB Image

GBX2 antibody detects GBX2 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GBX2 antibody (GTX129494) diluted by 1:1000.

Anti-GBX2 antibody used in Western Blot (WB). GTX129494

GTX129494 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GBX2 antibody (GTX129494) diluted at 1:1000.

Anti-GBX2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129494

GTX129494 ICC/IF Image

GBX2 antibody detects GBX2 protein at nucleus by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GBX2 protein stained by GBX2 antibody (GTX129494) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:50.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-GBX2 antibody used in Western Blot (WB). GTX129494

GTX129494 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GBX2 antibody (GTX129494) diluted at 1:1000.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169