Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GBX2 antibody (GTX129494) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GBX2 antibody detects GBX2 protein by western blot analysis. IMR32 whole cell extracts and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GBX2 antibody (GTX129494) diluted by 1:1000.

GBX2 antibody detects GBX2 protein at nucleus by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GBX2 protein stained by GBX2 antibody (GTX129494) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:50.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GBX2 antibody (GTX129494) diluted at 1:1000.

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GBX2 antibody (GTX129494) diluted at 1:1000.

GBX2 antibody detects GBX2 protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GBX2 antibody (GTX129494) diluted by 1:1000.

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GBX2 antibody (GTX129494) diluted at 1:1000.