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GCDFP15 antibody [PIP/1571]

Anti-GCDFP15 antibody [PIP/1571] used in Western Blot (WB). GTX34753
Anti-GCDFP15 antibody [PIP/1571] used in IHC (Paraffin sections) (IHC-P). GTX34753

Cat No. GTX34753

Host Mouse
Clonality Monoclonal
Clone Name PIP/1571
Isotype IgG2a
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5-1ug/ml
ICC/IF 0.5-1ug/ml
IHC-P 0.5-1ug/ml for 30 minutes at RT

Note :

IHC-P
Boiling tissue sections in 10mM Tris buffer with1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

17 kDa. ( Note )

Specificity/Sensitivity

It recognizes a protein of 15kDa, identified as Gross cystic disease fluid protein 15 (GCDFP-15). It is a major protein component of benign breast gross cysts. It is a known marker of breast cancer, as it is found in approximately 50% of all breast cancer specimens. GCDFP-15, also known as PIP, for prolactin inducible protein, is a prolactin and androgen controlled protein. This antibody is useful in the identification of metastatic breast carcinoma, or fluid analysis.
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant human GCDFP-15 protein fragment (aa 41-146) (exact sequence is proprietary)

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Prolactin Induced Protein,Gcdfp-15,Gcdfp15,Gpip4,Pip

Database

Research Area

DATA IMAGES
Anti-GCDFP15 antibody [PIP/1571] used in Western Blot (WB). GTX34753

GTX34753 WB Image

Western Blot Analysis (A) Human Pancreas (B) HepG2 Cell lysate Using GCDFP-15 Monoclonal Antibody (PIP/1571).

Anti-GCDFP15 antibody [PIP/1571] used in IHC (Paraffin sections) (IHC-P). GTX34753

GTX34753 IHC-P Image

Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with GCDFP-15 Monoclonal Antibody (PIP/1571).

Package List Price ($)
$ 329