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GCDH antibody

Anti-GCDH antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114427
Anti-GCDH antibody used in Western Blot (WB). GTX114427
Anti-GCDH antibody used in IHC (Paraffin sections) (IHC-P). GTX114427

Cat. No. GTX114427

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all GCDH products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

HepG2

Predict Reactivity

Rat, Bovine, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GCDH. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10619419

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glutaryl-CoA dehydrogenase , ACAD5 , GCD

Cellular Localization

Mitochondrion matrix

Background

The protein encoded by this gene belongs to the acyl-CoA dehydrogenase family. It catalyzes the oxidative decarboxylation of glutaryl-CoA to crotonyl-CoA and CO(2) in the degradative pathway of L-lysine, L-hydroxylysine, and L-tryptophan metabolism. It uses electron transfer flavoprotein as its electron acceptor. The enzyme exists in the mitochondrial matrix as a homotetramer of 45-kD subunits. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GCDH antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114427

GTX114427 ICC/IF Image

GCDH antibody detects GCDH protein at Mitochondria by immunofluorescent analysis.
Sample: HepG2 cells were fixed in -20ºC 100% MeOH for 5 min.
Green: GCDH protein stained by GCDH antibody (GTX114427) diluted at 1:500.
Blue: Hoechst 33343 staining.
Anti-GCDH antibody used in Western Blot (WB). GTX114427

GTX114427 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
10% SDS PAGE
GCD antibody
GTX114427 diluted at 1:1000

Anti-GCDH antibody used in IHC (Paraffin sections) (IHC-P). GTX114427

GTX114427 IHC-P Image

GCDH antibody detects GCDH protein at mitochondria on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
GCDH antibody (GTX114427) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169