GeneTex
United States (US)

GCH1 antibody, Internal

GTX88689_2102_WB_18122102_854.jpg

Cat No. GTX88689

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5-1.5μg/ml
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88689-PEP).

Specificity/Sensitivity

This antibody is expected to recognize all four isoforms (NP_000152.1 ; NP_001019195.1 ; NP_001019241.1 ; NP_001019242.1)

Predict Reactivity

Mouse, Rat(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-GKVHIGYLPNKQ, from the internal region of the protein sequence according to NP_000152.1 ; NP_001019195.1 ; NP_001019241.1 ; NP_001019242.1 .

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Gtp Cyclohydrolase 1,Dyt14,Dyt5,Dyt5A,Gch,Gtp-Ch-1,Gtpch1,Hpabh4B,Gch1

Background

This gene encodes a member of the GTP cyclohydrolase family. The encoded protein is the first and rate-limiting enzyme in tetrahydrobiopterin (BH4) biosynthesis, catalyzing the conversion of GTP into 7,8-dihydroneopterin triphosphate. BH4 is an essential cofactor required by aromatic amino acid hydroxylases as well as nitric oxide synthases. Mutations in this gene are associated with malignant hyperphenylalaninemia and dopa-responsive dystonia. Several alternatively spliced transcript variants encoding different isoforms have been described; however, not all variants give rise to a functional enzyme. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX88689_2102_WB_18122102_854.jpg

GTX88689 WB Image

WB analysis of human tonsil lysate using GTX88689 GCH1 antibody, Internal.
Dilution : 0.5µg/ml
Loading : 35µg protein in RIPA buffer

Package List Price ($)
$ 329