GeneTex
United States (US)

GCLC antibody, N-term

GTX16315_1769_IHC-P_18121410_167.jpg
GTX16315_1771_WB_18121410_966.jpg
GTX16315_1770_WB_18121410_935.jpg

Cat No. GTX16315

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.2-2.5 ug/ml
IHC-P 2-10 ug/ml
Not tested in other applications.

Calculated MW

73 kDa. ( Note )

Positive Control

human fetal liver , human liver , mouse heart

Predict Reactivity

Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 93%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 79%
PROPERTIES

Form

Liquid

Buffer

1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5-1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a N-terminal region of Human GCLC

Purification

Affinity Purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Glutamate-Cysteine Ligase Catalytic Subunit,Gcl,Gcs,Glcl,Glclc,Gclc

Background

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.[provided by RefSeq, Oct 2010]

Database

Research Area

DATA IMAGES
GTX16315_1769_IHC-P_18121410_167.jpg

GTX16315 IHC-P Image

IHC-P analysis of human bronchial epithelial tissue using GTX16315 GCLC antibody at 1:600. Left to right : DAPI, GCLC, Merge. Low pH, heat-induced antigen retrieval method utilizing Sodium Citrate buffer.

GTX16315_1771_WB_18121410_966.jpg

GTX16315 WB Image

WB analysis of human fetal liver tissue using GTX16315 GCLC antibody at 0.2-1μg/ml.

GTX16315_1770_WB_18121410_935.jpg

GTX16315 WB Image

WB analysis of human liver tissue using GTX16315 GCLC antibody at 1:1000.
Lane A : Primary antibody
Lane B : Primary antibody + Blocking peptide

REFERENCE
Application Reference
Package List Price ($)
$ 339