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GCLC antibody, N-term

Anti-GCLC antibody, N-term used in Western Blot (WB). GTX16315
Anti-GCLC antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX16315
Anti-GCLC antibody, N-term used in Western Blot (WB). GTX16315

Cat. No. GTX16315

Reference 18

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
50 μg ($419)
See all GCLC products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.2-2.5 ug/ml
IHC-P 2-10 ug/ml
Not tested in other applications.

Calculated MW

73 kDa. ( Note )

Positive Control

human fetal liver , human liver , mouse heart

PROPERTIES

Form

Liquid

Buffer

PBS, 2% Sucrose

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5-1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a N-terminal region of Human GCLC

Purification

Affinity Purified

Conjugation

Unconjugated

RRID

AB_2887531

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glutamate-cysteine ligase catalytic subunit , GCL , GCS , GLCL , GLCLC

Background

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.[provided by RefSeq, Oct 2010]

Database

Research Area

DATA IMAGES

Anti-GCLC antibody, N-term used in Western Blot (WB). GTX16315

GTX16315 WB Image

WB analysis of human fetal liver tissue using GTX16315 GCLC antibody at 0.2-1μg/ml.

Anti-GCLC antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX16315

GTX16315 IHC-P Image

IHC-P analysis of human bronchial epithelial tissue using GTX16315 GCLC antibody at 1:600. Left to right : DAPI, GCLC, Merge. Low pH, heat-induced antigen retrieval method utilizing Sodium Citrate buffer.

Anti-GCLC antibody, N-term used in Western Blot (WB). GTX16315

GTX16315 WB Image

WB analysis of human liver tissue using GTX16315 GCLC antibody at 1:1000.
Lane A : Primary antibody
Lane B : Primary antibody + Blocking peptide

REFERENCE

Application Reference

Idei U et al. Int J Mol Sci 2023; 24 (14)
Kanako Sendo et al. Oncotarget 2022; 13 : 224-236 Impact of the glutathione synthesis pathway on sulfasalazine-treated endometrial cancer.
Application : WB
Reactivity : Human
Hseu YC et al. Oxid Med Cell Longev 2020; 2020 : 2576823 The Antiaging Activity of Ergothioneine in UVA-Irradiated Human Dermal Fibroblasts via the Inhibition of the AP-1 Pathway and the Activation of Nrf2-Mediated Antioxidant Genes.
Application : WB
Reactivity : Human
Hseu YC et al. Oxid Med Cell Longev 2019; 2019 : 4098674 Zerumbone Exhibits Antiphotoaging and Dermatoprotective Properties in Ultraviolet A-Irradiated Human Skin Fibroblast Cells via the Activation of Nrf2/ARE Defensive Pathway.
Application : WB
Reactivity : Human
Huang SY et al. Biomolecules 2019; 9 (8) The Protective Effect of Hispidin against Hydrogen Peroxide-Induced Oxidative Stress in ARPE-19 Cells via Nrf2 Signaling Pathway.
Application : WB
Reactivity : Human
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REVIEW

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Application Reference
Idei U et al. Int J Mol Sci 2023; 24 (14)
Kanako Sendo et al. Oncotarget 2022; 13:224-236 Impact of the glutathione synthesis pathway on sulfasalazine-treated endometrial cancer.
Application : WB
Reactivity : Human
Hseu YC et al. Oxid Med Cell Longev 2020; 2020:2576823 The Antiaging Activity of Ergothioneine in UVA-Irradiated Human Dermal Fibroblasts via the Inhibition of the AP-1 Pathway and the Activation of Nrf2-Mediated Antioxidant Genes.
Application : WB
Reactivity : Human
Hseu YC et al. Oxid Med Cell Longev 2019; 2019:4098674 Zerumbone Exhibits Antiphotoaging and Dermatoprotective Properties in Ultraviolet A-Irradiated Human Skin Fibroblast Cells via the Activation of Nrf2/ARE Defensive Pathway.
Application : WB
Reactivity : Human
Huang SY et al. Biomolecules 2019; 9 (8) The Protective Effect of Hispidin against Hydrogen Peroxide-Induced Oxidative Stress in ARPE-19 Cells via Nrf2 Signaling Pathway.
Application : WB
Reactivity : Human
Wu CT et al. Oxid Med Cell Longev 2019; 2019:9056845 Salvianolic Acid C against Acetaminophen-Induced Acute Liver Injury by Attenuating Inflammation, Oxidative Stress, and Apoptosis through Inhibition of the Keap1/Nrf2/HO-1 Signaling.
Application : WB
Reactivity : Mouse
Hseu YC et al. Biochem Pharmacol 2019; 164:299-310 The in vitro and in vivo depigmenting activity of Coenzyme Q10 through the down-regulation of α-MSH signaling pathways and induction of Nrf2/ARE-mediated antioxidant genes in UVA-irradiated skin keratinocytes.
Application : WB
Reactivity : Human
Hseu YC et al. J Dermatol Sci 2018; (Epub) Trans-cinnamic acid attenuates UVA-induced photoaging through inhibition of AP-1 activation and induction of Nrf2-mediated antioxidant genes in human skin fibroblasts.
Application : WB
Reactivity : Human
Wu YL et al. Free Radic Biol Med 2018; 115:309-317 Caffeic acid and resveratrol ameliorate cellular damage in cell and Drosophila models of spinocerebellar ataxia type 3 through upregulation of Nrf2 pathway.
Application : WB
Reactivity : Human
Yang HL et al. Biochem Pharmacol 2017; 148:130-146 Zerumbone protects human skin keratinocytes against UVA-irradiated damages through Nrf2 induction.
Application : WB
Reactivity : Human
Chang HC et al. Oxid Med Cell Longev. 2016; 2016:8257238 Hericium erinaceus Inhibits TNF-α-Induced Angiogenesis and ROS Generation through Suppression of MMP-9/NF-κB Signaling and Activation of Nrf2-Mediated Antioxidant Genes in Human EA.hy926 Endothelial Cells.
Application : WB
Reactivity : Human
Chen YH et al. PLoS One 2016; 11 (4):e0154210 Caveolin-1 Expression Ameliorates Nephrotic Damage in a Rabbit Model of Cholesterol-Induced Hypercholesterolemia.
Application : WB, IHC-P
Hseu YC et al. journal of functional foods 2015; 12:303-318 The dermato-protective effects of lucidone from Lindera erythrocarpa through the induction of Nrf2-mediated antioxidant genes in UVA-irradiated human skin keratinocytes.
Application : WB
Reactivity : Human
Hseu YC et al. Free Radic Biol Med 2015; 86:102-17 Dermato-protective properties of ergothioneine through induction of Nrf2/ARE-mediated antioxidant genes in UVA-irradiated Human keratinocytes.
Application : WB
Reactivity : Human
Yang HL et al. Biochemical Pharmacology 2015;:144-156 Anti-angiogenic properties of coenzyme Q0 through downregulation of MMP-9/NF-kB and upregulation of HO-1 signaling in TNF-a-activated human endothelial cells.
Application : WB
Reactivity : Human
Urrunaga NH et al. Free Radic Biol Med 2015; 78:66-81 M1 muscarinic receptors modify oxidative stress response to acetaminophen-induced acute liver injury.
Application : WB
Reactivity : Mouse
Rachakonda V et al. Sci Rep 2015; 5:14110 M1 Muscarinic Receptor Deficiency Attenuates Azoxymethane-Induced Chronic Liver Injury in Mice.
Application : IHC-P
Reactivity : Mouse
Yang HL et al. Food & Function 2014; 6:229-240 Induction of Nrf2-mediated genes by Antrodia salmonea inhibits ROS generation and inflammatory effects in lipopolysaccharidestimulated RAW264.7 macrophages.
Application : WB
Reactivity : Mouse
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 419