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GCLC antibody [N1N3]

Orthogonal
Anti-GCLC antibody [N1N3] used in Western Blot (WB). GTX113197
Anti-GCLC antibody [N1N3] used in Western Blot (WB). GTX113197
Anti-GCLC antibody [N1N3] used in Western Blot (WB). GTX113197

Cat. No. GTX113197

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all GCLC products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
Not tested in other applications.

Calculated MW

73 kDa. ( Note )

Predict Reactivity

Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GCLC. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10617941

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glutamate-cysteine ligase catalytic subunit , GCL , GCS , GLCL , GLCLC

Background

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. The gene encoding the catalytic subunit encodes a protein of 367 amino acids with a calculated molecular weight of 72.773 kDa and maps to chromosome 6. The regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Deficiency of gamma-glutamylcysteine synthetase in human is associated with enzymopathic hemolytic anemia. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GCLC antibody [N1N3] used in Western Blot (WB). GTX113197

GTX113197 WB Image

Orthogonal

The data was published in the journal Oxid Med Cell Longev in 2019. PMID: 31534619

Anti-GCLC antibody [N1N3] used in Western Blot (WB). GTX113197

GTX113197 WB Image

The data was published in the journal Oxid Med Cell Longev in 2019. PMID: 31534619

Anti-GCLC antibody [N1N3] used in Western Blot (WB). GTX113197

GTX113197 WB Image

The data was published in the journal Oxid Med Cell Longev in 2019. PMID: 31534619

REFERENCE

Application Reference

Bai X et al. Oxid Med Cell Longev 2019; 2019 : 2432416 Sesamin Enhances Nrf2-Mediated Protective Defense against Oxidative Stress and Inflammation in Colitis via AKT and ERK Activation.
Application : WB
Reactivity : Human
Jeongsu Han et al. Oxidative Medicine and Cellular Longevity 2019; 2019 : Article ID 2432416 Sesamin Enhances Nrf2-Mediated Protective Defense against Oxidative Stress and Inflammation in Colitis via AKT and ERK Activation.
Application : WB
Reactivity : Human
Chen YH et al. Mol Med Rep 2017; (Epub) H2O2 induces caveolin-1 degradation and impaired mitochondrial function in E11 podocytes.
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169