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GCLM antibody [N1N3]

Anti-GCLM antibody [N1N3] used in Western Blot (WB). GTX111858
Anti-GCLM antibody [N1N3] used in Western Blot (WB). GTX111858
Anti-GCLM antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX111858
Anti-GCLM antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111858

Cat No. GTX111858

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Positive Control

HeLa , 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human GCLM. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

glutamate-cysteine ligase modifier subunit , GLCLR

Background

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GCLM antibody [N1N3] used in Western Blot (WB). GTX111858

GTX111858 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with GCLM antibody [N1N3] (GTX111858) diluted at 1:7000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GCLM antibody [N1N3] used in Western Blot (WB). GTX111858

GTX111858 WB Image

Sample (30 μg of whole cell lysate)
A: H1299
12% SDS PAGE
GTX111858 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GCLM antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX111858

GTX111858 IHC-P Image

Immunohistochemical analysis of paraffin-embedded BT474 xenograft, using GCLM(GTX111858) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GCLM antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111858

GTX111858 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using GCLM(GTX111858) antibody at 1:200 dilution.

REFERENCE

There are currently no references for GCLM antibody [N1N3] (GTX111858). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for GCLM antibody [N1N3] (GTX111858). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169