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GCLM antibody [N1N3]

KO/KD
Anti-GCLM antibody [N1N3] used in Western Blot (WB). GTX111858
Anti-GCLM antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX111858
Anti-GCLM antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111858
Anti-GCLM antibody [N1N3] used in Western Blot (WB). GTX111858

Cat. No. GTX111858

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all GCLM products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Positive Control

H1299 , HeLa

Predict Reactivity

Mouse, Rat, Bovine, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human GCLM. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950358

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glutamate-cysteine ligase modifier subunit , GLCLR

Background

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GCLM antibody [N1N3] used in Western Blot (WB). GTX111858

GTX111858 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with GCLM antibody [N1N3] (GTX111858) diluted at 1:7000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GCLM antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX111858

GTX111858 IHC-P Image

Immunohistochemical analysis of paraffin-embedded BT474 xenograft, using GCLM(GTX111858) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GCLM antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111858

GTX111858 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using GCLM(GTX111858) antibody at 1:200 dilution.

Anti-GCLM antibody [N1N3] used in Western Blot (WB). GTX111858

GTX111858 WB Image

Sample (30 μg of whole cell lysate)
A: H1299
12% SDS PAGE
GTX111858 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169