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GCLM antibody

Anti-GCLM antibody used in Western Blot (WB). GTX114075
Anti-GCLM antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114075
Anti-GCLM antibody used in Western Blot (WB). GTX114075
Anti-GCLM antibody used in Western Blot (WB). GTX114075
Anti-GCLM antibody used in Western Blot (WB). GTX114075
Anti-GCLM antibody used in Western Blot (WB). GTX114075

Cat No. GTX114075

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Positive Control

HeLa , A431 , mouse liver , rat liver , rat kidney

Predict Reactivity

Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.33 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GCLM. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

glutamate-cysteine ligase modifier subunit , GLCLR

Background

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-GCLM antibody used in Western Blot (WB). GTX114075

GTX114075 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with GCLM antibody (GTX114075) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GCLM antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114075

GTX114075 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using GCLM(GTX114075) antibody at 1:200 dilution.

Anti-GCLM antibody used in Western Blot (WB). GTX114075

GTX114075 WB Image

Mouse tissue extract (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with GCLM antibody (GTX114075) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GCLM antibody used in Western Blot (WB). GTX114075

GTX114075 WB Image

Sample (50 µg of whole cell lysate)
A: rat liver
12% SDS PAGE
GTX114075 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GCLM antibody used in Western Blot (WB). GTX114075

GTX114075 WB Image

Sample (30 µg of whole cell lysate)
A: A431 (GTX27909)
12% SDS PAGE
GCLM antibody
GTX114075 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GCLM antibody used in Western Blot (WB). GTX114075

GTX114075 WB Image

Rat tissue extract (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with GCLM antibody (GTX114075) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW
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Application Reference
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169