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GCLM antibody

KO/KD Orthogonal
Anti-GCLM antibody used in Western Blot (WB). GTX114075
Anti-GCLM antibody used in Western Blot (WB). GTX114075
Anti-GCLM antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114075
Anti-GCLM antibody used in Western Blot (WB). GTX114075
Anti-GCLM antibody used in Western Blot (WB). GTX114075
Anti-GCLM antibody used in Western Blot (WB). GTX114075
Anti-GCLM antibody used in Western Blot (WB). GTX114075

Cat. No. GTX114075

Reference 13
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all GCLM products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Positive Control

rat liver , mouse liver , rat kidney , HeLa

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.21 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GCLM. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10619535

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glutamate-cysteine ligase modifier subunit , GLCLR

Background

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GCLM antibody used in Western Blot (WB). GTX114075

GTX114075 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with GCLM antibody (GTX114075) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GCLM antibody used in Western Blot (WB). GTX114075

GTX114075 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with GCLM antibody (GTX114075) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GCLM antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114075

GTX114075 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using GCLM(GTX114075) antibody at 1:200 dilution.

Anti-GCLM antibody used in Western Blot (WB). GTX114075

GTX114075 WB Image

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with GCLM antibody (GTX114075) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GCLM antibody used in Western Blot (WB). GTX114075

GTX114075 WB Image

Sample (50 μg of whole cell lysate)
A: rat liver
12% SDS PAGE
GTX114075 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GCLM antibody used in Western Blot (WB). GTX114075

GTX114075 WB Image

Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with GCLM antibody (GTX114075) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GCLM antibody used in Western Blot (WB). GTX114075

GTX114075 WB Image

Orthogonal

The data was published in the journal Biomolecules in 2019. PMID: 31443530

REFERENCE

Application Reference

Yoon SJ et al. Cancer Res 2023; 83 (9) : 1426-1442 Comprehensive Metabolic Tracing Reveals the Origin and Catabolism of Cysteine in Mammalian Tissues and Tumors.
Giovanni C Forcina et al. Proc Natl Acad Sci U S A 2022; 119 (11) : e2118646119 Ferroptosis regulation by the NGLY1/NFE2L1 pathway.
Application : WB
Reactivity : Human
Jesse Rabinowitz et al. Virology 2021; 556 : 149-160 xCT/SLC7A11 antiporter function inhibits HIV-1 infection.
Application : WB
Reactivity : Human
NandyMazumdar M et al. Biochem J 2021; 478 (20) : 3741-3756 BACH1, the master regulator of oxidative stress, has a dual effect on CFTR expression.
Application : WB
Hseu YC et al. Redox Biol 2021; 44 : 102007 The in vitro and in vivo depigmenting activity of pterostilbene through induction of autophagy in melanocytes and inhibition of UVA-irradiated α-MSH in keratinocytes via Nrf2-mediated antioxidant pathways.
Application : WB
Reactivity : Human
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Submit a Reference

REVIEW

GCLM antibody Cat. No. GTX114075

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-GCLM antibody used in Western Blot (WB). GTX114075
Date : Anonymous submitted on 25-Apr-2023
Score :
Application Tested : WB
Sample Species : Ms
Sample : mouse liver tissue lysate
Amount used : 40μg
Blocking : 5% non-fat milk in TBST, 25°C, 1Hr
Primary Antibody : 1:2000, 4°C, >12Hr
Submit a Review
Application Reference
Yoon SJ et al. Cancer Res 2023; 83 (9):1426-1442 Comprehensive Metabolic Tracing Reveals the Origin and Catabolism of Cysteine in Mammalian Tissues and Tumors.
Giovanni C Forcina et al. Proc Natl Acad Sci U S A 2022; 119 (11):e2118646119 Ferroptosis regulation by the NGLY1/NFE2L1 pathway.
Application : WB
Reactivity : Human
Jesse Rabinowitz et al. Virology 2021; 556:149-160 xCT/SLC7A11 antiporter function inhibits HIV-1 infection.
Application : WB
Reactivity : Human
NandyMazumdar M et al. Biochem J 2021; 478 (20):3741-3756 BACH1, the master regulator of oxidative stress, has a dual effect on CFTR expression.
Application : WB
Hseu YC et al. Redox Biol 2021; 44:102007 The in vitro and in vivo depigmenting activity of pterostilbene through induction of autophagy in melanocytes and inhibition of UVA-irradiated α-MSH in keratinocytes via Nrf2-mediated antioxidant pathways.
Application : WB
Reactivity : Human
Hori Y et al. Cells 2021; 10 (5) Aromatic-Turmerone Analogs Protect Dopaminergic Neurons in Midbrain Slice Cultures through Their Neuroprotective Activities.
Application : WB
Reactivity : Mouse
Huang SY et al. Biomolecules 2019; 9 (8) The Protective Effect of Hispidin against Hydrogen Peroxide-Induced Oxidative Stress in ARPE-19 Cells via Nrf2 Signaling Pathway.
Application : WB
Reactivity : Human
Rojas-Morales P et al. Biomolecules 2019; 9 (9) Mechanisms of Fasting-Mediated Protection against Renal Injury and Fibrosis Development after Ischemic Acute Kidney Injury.
Application : WB
Reactivity : Rat
Wu CT et al. Oxid Med Cell Longev 2019; 2019:9056845 Salvianolic Acid C against Acetaminophen-Induced Acute Liver Injury by Attenuating Inflammation, Oxidative Stress, and Apoptosis through Inhibition of the Keap1/Nrf2/HO-1 Signaling.
Application : WB
Reactivity : Mouse
Wu YL et al. Free Radic Biol Med 2018; 115:309-317 Caffeic acid and resveratrol ameliorate cellular damage in cell and Drosophila models of spinocerebellar ataxia type 3 through upregulation of Nrf2 pathway.
Application : WB
Reactivity : Human
Choi EJ et al. Oncogene 2017; (Epub) A clinical drug library screen identifies * propionate as an NRF2 inhibitor with potential therapeutic efficacy in KEAP1 mutant lung cancer.
Application : WB
Reactivity : Human
Furuya AK et al. PLoS Pathog 2016; 12 (4):e1005581 Sulforaphane Inhibits HIV Infection of Macrophages through Nrf2.
Application : WB
Reactivity : Human
Liu XM et al. Am J Physiol Heart Circ Physiol 2013; 304 (12):H1634-43 Physiological cyclic strain promotes endothelial cell survival via the induction of heme oxygenase-1.
Application : WB
Reactivity : Human
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169