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GCLM antibody

Anti-GCLM antibody used in Western Blot (WB). GTX32622
Anti-GCLM antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32622

Cat No. GTX32622

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse
Package
100 μl ($329)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
Not tested in other applications.

Calculated MW

31 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-274 of human GCLM (NP_002052.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

glutamate-cysteine ligase modifier subunit , GLCLR

Background

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015]

Database

Research Area

DATA IMAGES
Anti-GCLM antibody used in Western Blot (WB). GTX32622

GTX32622 WB Image

WB analysis of various samples using GTX32622 GCLM antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-GCLM antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32622

GTX32622 ICC/IF Image

ICC/IF analysis of U2OS cell using GTX32622 GCLM antibody.
Blue : DAPI

REFERENCE
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REVIEW
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Package List Price ($)
$ 329