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GCN2 antibody [ACAA-5]

Anti-GCN2 antibody [ACAA-5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57742
Anti-GCN2 antibody [ACAA-5] used in Flow cytometry (FACS). GTX57742
Anti-GCN2 antibody [ACAA-5] used in Western Blot (WB). GTX57742
Anti-GCN2 antibody [ACAA-5] used in IHC (Paraffin sections) (IHC-P). GTX57742

Cat. No. GTX57742

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 ACAA-5

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS, IHC

Reactivity

 Human
Package
100 μg ($499)
See all GCN2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
ICC/IF 1:50-1:200
IHC-P Assay dependent
FACS 1:100
IHC 1:50-1:200
Not tested in other applications.

Calculated MW

187 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5-1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human GCN2.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

EIF2AK4 , GCN2 , PVOD2 , eukaryotic translation initiation factor 2 alpha kinase 4

Cellular Localization

Cytoplasm

Background

This gene encodes a member of a family of kinases that phosphorylate the alpha subunit of eukaryotic translation initiation factor-2 (EIF2), resulting in the downregulaton of protein synthesis. The encoded protein responds to amino acid deprivation by binding uncharged transfer RNAs. It may also be activated by glucose deprivation and viral infection. Mutations in this gene have been found in individuals suffering from autosomal recessive pulmonary venoocclusive-disease-2. [provided by RefSeq, Mar 2014]

Database

Research Area

DATA IMAGES

Anti-GCN2 antibody [ACAA-5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57742

GTX57742 ICC/IF Image

ICC/IF analysis of A549 cells using GTX57742 GCN2 antibody [ACAA-5].
Green : Primary antibody
Blue : DAPI
Fixation : 4% PFA
Permeabilization : 0.25% Triton X100/PBS.

Anti-GCN2 antibody [ACAA-5] used in Flow cytometry (FACS). GTX57742

GTX57742 FACS Image

FACS analysis of MCF-7 cells using GTX57742 GCN2 antibody [ACAA-5].
Black : Blank
Red : Primary antibody
Dilution : 1:100

Anti-GCN2 antibody [ACAA-5] used in Western Blot (WB). GTX57742

GTX57742 WB Image

WB analysis of HeLa whole cell lysate using GTX57742 GCN2 antibody [ACAA-5].
Dilution : 0.5μg/ml
Loading : 50μg

Anti-GCN2 antibody [ACAA-5] used in IHC (Paraffin sections) (IHC-P). GTX57742

GTX57742 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX57742 GCN2 antibody [ACAA-5].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 499