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GCN2 antibody

Anti-GCN2 antibody used in Western Blot (WB). GTX57741
Anti-GCN2 antibody used in IHC (Paraffin sections) (IHC-P). GTX57741
Anti-GCN2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57741
Anti-GCN2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57741
Anti-GCN2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57741
Anti-GCN2 antibody used in IHC (Paraffin sections) (IHC-P). GTX57741

Cat. No. GTX57741

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all GCN2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

187 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human GCN2 (NP_001013725.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887953

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

eukaryotic translation initiation factor 2 alpha kinase 4 , GCN2 , PVOD2

Cellular Localization

Cytoplasm

Background

This gene encodes a member of a family of kinases that phosphorylate the alpha subunit of eukaryotic translation initiation factor-2 (EIF2), resulting in the downregulaton of protein synthesis. The encoded protein responds to amino acid deprivation by binding uncharged transfer RNAs. It may also be activated by glucose deprivation and viral infection. Mutations in this gene have been found in individuals suffering from autosomal recessive pulmonary venoocclusive-disease-2. [provided by RefSeq, Mar 2014]

Database

Research Area

DATA IMAGES

Anti-GCN2 antibody used in Western Blot (WB). GTX57741

GTX57741 WB Image

WB analysis of HepG2 cell lysate using GTX57741 GCN2 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-GCN2 antibody used in IHC (Paraffin sections) (IHC-P). GTX57741

GTX57741 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX57741 GCN2 antibody.
Antigen retrieval : 10 mM citrate buffer pH 6.0
Dilution : 1:200

Anti-GCN2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57741

GTX57741 ICC/IF Image

ICC/IF analysis of NIH-3T3 cells using GTX57741 GCN2 antibody.
Red : Primary antibody
Blue : DAPI
Dilution : 1:200

Anti-GCN2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57741

GTX57741 ICC/IF Image

ICC/IF analysis of PC-12 cells using GTX57741 GCN2 antibody.
Red : Primary antibody
Blue : DAPI
Dilution : 1:200

Anti-GCN2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57741

GTX57741 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX57741 GCN2 antibody.
Red : Primary antibody
Blue : DAPI
Dilution : 1:200

Anti-GCN2 antibody used in IHC (Paraffin sections) (IHC-P). GTX57741

GTX57741 IHC-P Image

IHC-P analysis of rat ovary tissue using GTX57741 GCN2 antibody.
Antigen retrieval : 10 mM citrate buffer pH 6.0
Dilution : 1:200

REFERENCE

Application Reference

Wang IH et al. Cancers (Basel) 2020; 12 (5) Mevalonate Pathway Enzyme HMGCS1 Contributes to Gastric Cancer Progression.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399