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GCN2 (phospho Thr899) antibody

Anti-GCN2 (phospho Thr899) antibody used in Flow cytometry (FACS). GTX04587
Anti-GCN2 (phospho Thr899) antibody used in Flow cytometry (FACS). GTX04587

Cat. No. GTX04587

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, FACS

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all GCN2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:300-1:5000
FACS 1:20-1:100
Not tested in other applications.

Calculated MW

187 kDa. ( Note )

Product Note

This phosphorylation site is homologous to Thr898 in Mouse and Thr899 in Rat.

PROPERTIES

Form

Liquid

Buffer

0.01M TBS pH 7.4, 1% BSA, 50% Glycerol

Preservative

0.03% Proclin300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic phosphopeptide derived from human GCN2 around the phosphorylation site of Thr899.

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

eukaryotic translation initiation factor 2 alpha kinase 4 , GCN2 , PVOD2

Cellular Localization

Cytoplasm

Background

This gene encodes a member of a family of kinases that phosphorylate the alpha subunit of eukaryotic translation initiation factor-2 (EIF2), resulting in the downregulaton of protein synthesis. The encoded protein responds to amino acid deprivation by binding uncharged transfer RNAs. It may also be activated by glucose deprivation and viral infection. Mutations in this gene have been found in individuals suffering from autosomal recessive pulmonary venoocclusive-disease-2. [provided by RefSeq, Mar 2014]

Database

Research Area

DATA IMAGES

Anti-GCN2 (phospho Thr899) antibody used in Flow cytometry (FACS). GTX04587

GTX04587 FACS Image

FACS analysis of 4% PFA-fixed Molt-4 cells using GTX04587 GCN2 (phospho Thr899) antibody.
Green : Primary antibody
Orange : Isotype control
Blue : Secondary antibody only
Black : Unlabeled cells
Permeabilization : 90% ice-cold methanol
Dilution : 1:50

Anti-GCN2 (phospho Thr899) antibody used in Flow cytometry (FACS). GTX04587

GTX04587 FACS Image

FACS analysis of 4% PFA-fixed mouse spleen cells using GTX04587 GCN2 (phospho Thr899) antibody.
Green : Primary antibody
Orange : Isotype control
Blue : Secondary antibody only
Black : Unlabeled cells
Permeabilization : 0.1% PBST
Dilution : 1:50

REFERENCE

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REVIEW

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Package List Price ($)
$ 399