FACS analysis of 4% PFA-fixed mouse spleen cells using GTX04587 GCN2 (phospho Thr899) antibody.
Green : Primary antibody
Orange : Isotype control
Blue : Secondary antibody only
Black : Unlabeled cells
Permeabilization : 0.1% PBST
Dilution : 1:50

FACS analysis of 4% PFA-fixed Molt-4 cells using GTX04587 GCN2 (phospho Thr899) antibody.
Green : Primary antibody
Orange : Isotype control
Blue : Secondary antibody only
Black : Unlabeled cells
Permeabilization : 90% ice-cold methanol
Dilution : 1:50