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GCN2 (phospho Thr899) antibody

Anti-GCN2 (phospho Thr899) antibody used in IHC (Paraffin sections) (IHC-P). GTX04588
Anti-GCN2 (phospho Thr899) antibody used in IHC (Paraffin sections) (IHC-P). GTX04588
Anti-GCN2 (phospho Thr899) antibody used in ELISA (ELISA). GTX04588
Anti-GCN2 (phospho Thr899) antibody used in IHC (Paraffin sections) (IHC-P). GTX04588

Cat. No. GTX04588

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all GCN2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:3000
IHC-P 1:50-1:200
ELISA Assay dependent
Not tested in other applications.

Calculated MW

187 kDa. ( Note )

Predict Reactivity

Rabbit, Sheep, Bovine, Dog, Chicken, Pig, Horse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS pH 7.4, 150 mM NaCl, 50% glycerol

Preservative

0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human EIF2AK4(Accession Q9P2K8), corresponding to amino acid residues around phosphorylated Thr899.

Purification

Purified by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

eukaryotic translation initiation factor 2 alpha kinase 4 , GCN2 , PVOD2

Cellular Localization

Cytoplasm

Background

This gene encodes a member of a family of kinases that phosphorylate the alpha subunit of eukaryotic translation initiation factor-2 (EIF2), resulting in the downregulaton of protein synthesis. The encoded protein responds to amino acid deprivation by binding uncharged transfer RNAs. It may also be activated by glucose deprivation and viral infection. Mutations in this gene have been found in individuals suffering from autosomal recessive pulmonary venoocclusive-disease-2. [provided by RefSeq, Mar 2014]

Database

Research Area

DATA IMAGES

Anti-GCN2 (phospho Thr899) antibody used in IHC (Paraffin sections) (IHC-P). GTX04588

GTX04588 IHC-P Image

IHC-P analysis of rat lung tissue using GTX04588 GCN2 (phospho Thr899) antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:200

Anti-GCN2 (phospho Thr899) antibody used in IHC (Paraffin sections) (IHC-P). GTX04588

GTX04588 IHC-P Image

IHC-P analysis of human liver cancer tissue using GTX04588 GCN2 (phospho Thr899) antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:200

Anti-GCN2 (phospho Thr899) antibody used in ELISA (ELISA). GTX04588

GTX04588 ELISA Image

ELISA analysis of phospho- and non phosho- immunogen peptide using serial diluted GTX04588 GCN2 (phospho Thr899) antibody.
P-peptide : phospho-peptide
N-peptide : non-phospho-peptide
Peptides concentration : 1 μg/mL

Anti-GCN2 (phospho Thr899) antibody used in IHC (Paraffin sections) (IHC-P). GTX04588

GTX04588 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX04588 GCN2 (phospho Thr899) antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:200

REFERENCE

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REVIEW

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Package List Price ($)
$ 399