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GCN5 antibody [AT3G13]

Anti-GCN5 antibody [AT3G13] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50005
Anti-GCN5 antibody [AT3G13] used in Western Blot (WB). GTX50005

Cat No. GTX50005

Host

Mouse

Clonality

Monoclonal

Clone Name

AT3G13

Isotype

IgG2a

Application

WB, ICC/IF, ELISA

Reactivity

Human
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

94 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

In Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human GCN5 (411-837aa) purified from E. coli

Purification

By protein-G affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Lysine Acetyltransferase 2A , Gcn5 , Gcn5L2 , Pcaf-B , Hgcn5 , Kat2A

Background

GCN5L2, as known as GCN5 and KAT2A is the use of immunoprecipitated GCN5L2 for acetylation to provide the additional components important for acetylation. GCN5L2 can be one of the potential enzymes involved in acetylation in vivo. GCN5L2 is expressed predominantly in the embryo and newborn and is essential for normal embryonic development. Therefore, GCN5L2 appears to be important for differentiation of embryonic-derived preadipocytes. On the one hand, loss of GCN5L2 leads to high incidence of apoptosis in the GCN5L2 mutants that begins before the onset of morphological abnormality.

Database

Research Area

DATA IMAGES

Anti-GCN5 antibody [AT3G13] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50005

GTX50005 ICC/IF Image

Immunofluorescence of HeLa cells were stained by monoclonal anti-human GCN5L2 antibody (1:500) with Alexa 488 (Green).

Anti-GCN5 antibody [AT3G13] used in Western Blot (WB). GTX50005

GTX50005 WB Image

Cell lysates of HeLa (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human GCN5L2 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf