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GCN5 antibody [AT3G13]

Anti-GCN5 antibody [AT3G13] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50005
Anti-GCN5 antibody [AT3G13] used in Western Blot (WB). GTX50005

Cat. No. GTX50005

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 AT3G13

Isotype

 IgG2a

Application

 WB, ICC/IF, ELISA

Reactivity

 Human
Package
100 μl ($399)
See all GCN5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

94 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human GCN5 (411-837aa) purified from E. coli

Purification

By protein-G affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

lysine acetyltransferase 2A , GCN5 , GCN5L2 , PCAF-b , hGCN5

Cellular Localization

Nucleus

Background

KAT2A, or GCN5, is a histone acetyltransferase (HAT) that functions primarily as a transcriptional activator. It also functions as a repressor of NF-kappa-B (see MIM 164011) by promoting ubiquitination of the NF-kappa-B subunit RELA (MIM 164014) in a HAT-independent manner (Mao et al., 2009 [PubMed 19339690]).[supplied by OMIM, Sep 2009]

Database

Research Area

DATA IMAGES

Anti-GCN5 antibody [AT3G13] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50005

GTX50005 ICC/IF Image

Immunofluorescence of HeLa cells were stained by monoclonal anti-human GCN5L2 antibody (1:500) with Alexa 488 (Green).

Anti-GCN5 antibody [AT3G13] used in Western Blot (WB). GTX50005

GTX50005 WB Image

Cell lysates of HeLa (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human GCN5L2 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399