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GCN5 antibody [C2C3], C-term

Orthogonal
Anti-GCN5 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX114428
Anti-GCN5 antibody [C2C3], C-term used in Western Blot (WB). GTX114428
Anti-GCN5 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114428
Anti-GCN5 antibody [C2C3], C-term used in Western Blot (WB). GTX114428
Anti-GCN5 antibody [C2C3], C-term used in Western Blot (WB). GTX114428

Cat. No. GTX114428

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all GCN5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

94 kDa. ( Note )

Positive Control

NIH-3T3 , JC , BCL-1

Product Note

This antibody was raised against KAT2A protein, and based on sequence homology, it may cross react with KAT2B protein.

Predict Reactivity

Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human GCN5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11164592

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

lysine acetyltransferase 2A , GCN5 , GCN5L2 , PCAF-b , hGCN5

Cellular Localization

Nucleus

Background

KAT2A, or GCN5, is a histone acetyltransferase (HAT) that functions primarily as a transcriptional activator. It also functions as a repressor of NF-kappa-B (see MIM 164011) by promoting ubiquitination of the NF-kappa-B subunit RELA (MIM 164014) in a HAT-independent manner (Mao et al., 2009 [PubMed 19339690]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-GCN5 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX114428

GTX114428 IHC-P Image

GCN5 antibody [C2C3], C-term detects GCN5 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
GCN5 stained by GCN5 antibody [C2C3], C-term (GTX114428) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GCN5 antibody [C2C3], C-term used in Western Blot (WB). GTX114428

GTX114428 WB Image

Orthogonal

293T whole cell and nuclear extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GCN5 antibody [C2C3], C-term (GTX114428) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GCN5 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114428

GTX114428 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed MCF-7, using GCN5(GTX114428) antibody at 1:500 dilution.

Anti-GCN5 antibody [C2C3], C-term used in Western Blot (WB). GTX114428

GTX114428 WB Image

Sample (30 μg of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
7.5% SDS PAGE
GTX114428 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GCN5 antibody [C2C3], C-term used in Western Blot (WB). GTX114428

GTX114428 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GCN5 antibody [C2C3], C-term (GTX114428) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Wang SA et al. Sci Rep 2020; 10 (1) : 20870 USP24 stabilizes bromodomain containing proteins to promote lung cancer malignancy.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169