GeneTex
United States (US)

GCN5 antibody [C3], C-term

GTX100286_WB_19011506_293.jpg
GTX100286_39443_20160113_IHC-P_R_19011506_623.jpg

Cat No. GTX100286

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

94 kDa. ( Note )

Positive Control

A431 , H1299 , HeLa

Predict Reactivity

Mouse, Chicken(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human GCN5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Lysine Acetyltransferase 2A,Gcn5,Gcn5L2,Pcaf-B,Hgcn5,Kat2A

Cellular Localization

Nucleus

Background

KAT2A, or GCN5, is a histone acetyltransferase (HAT) that functions primarily as a transcriptional activator. It also functions as a repressor of NF-kappa-B (see MIM 164011) by promoting ubiquitination of the NF-kappa-B subunit RELA (MIM 164014) in a HAT-independent manner (Mao et al., 2009 [PubMed 19339690]).[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX100286_WB_19011506_293.jpg

GTX100286 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
B: H1299
C: Hela
7.5% SDS PAGE
GCN5 antibody
GTX100286 diluted at 1:1000

GTX100286_39443_20160113_IHC-P_R_19011506_623.jpg

GTX100286 IHC-P Image

GCN5 antibody [C3], C-term detects GCN5 protein at nucleus and cytoplasm in rat testis by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
GCN5 antibody [C3], C-term (GTX100286) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169