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GCNF antibody [4G8]

Anti-GCNF antibody [4G8] used in IHC (Paraffin sections) (IHC-P). GTX60623
Anti-GCNF antibody [4G8] used in Flow cytometry (FACS). GTX60623
Anti-GCNF antibody [4G8] used in ELISA (ELISA). GTX60623
Anti-GCNF antibody [4G8] used in IHC (Paraffin sections) (IHC-P). GTX60623
Anti-GCNF antibody [4G8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60623
Anti-GCNF antibody [4G8] used in Western Blot (WB). GTX60623
Anti-GCNF antibody [4G8] used in Western Blot (WB). GTX60623

Cat. No. GTX60623

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 4G8

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS, ELISA

Reactivity

 Human
Package
100 μg ($409)
See all GCNF products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/400
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant fragment of human GCNF (AA: 65-118) expressed in E. Coli.

Purification

Protein G Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nuclear receptor subfamily 6 group A member 1 , CT150 , GCNF , GCNF1 , NR61 , RTR , hGCNF , hRTR

Cellular Localization

Nucleus

Background

This gene encodes an orphan nuclear receptor which is a member of the nuclear hormone receptor family. Its expression pattern suggests that it may be involved in neurogenesis and germ cell development. The protein can homodimerize and bind DNA, but in vivo targets have not been identified. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Jun 2013]

Database

Research Area

DATA IMAGES

Anti-GCNF antibody [4G8] used in IHC (Paraffin sections) (IHC-P). GTX60623

GTX60623 IHC-P Image

IHC-P analysis of rectum cancer tissue using GTX60623 GCNF antibody [4G8].

Anti-GCNF antibody [4G8] used in Flow cytometry (FACS). GTX60623

GTX60623 FACS Image

FACS analysis of K562 cells using GTX60623 GCNF antibody [4G8].
Green : GCNF
Red : negative control

Anti-GCNF antibody [4G8] used in ELISA (ELISA). GTX60623

GTX60623 ELISA Image

ELISA analysis of antigen using GTX60623 GCNF antibody [4G8].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

Anti-GCNF antibody [4G8] used in IHC (Paraffin sections) (IHC-P). GTX60623

GTX60623 IHC-P Image

IHC-P analysis of human cerebellum tissue using GTX60623 GCNF antibody [4G8].

Anti-GCNF antibody [4G8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60623

GTX60623 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX60623 GCNF antibody [4G8].
Green : GCNF
Blue: DRAQ5 fluorescent DNA dye

Anti-GCNF antibody [4G8] used in Western Blot (WB). GTX60623

GTX60623 WB Image

WB analysis of K562 (1), NTERA-2 (2), HEK293 (3), HUVE-12 (4), and HeLa (5) cell lysate using GTX60623 GCNF antibody [4G8].

Anti-GCNF antibody [4G8] used in Western Blot (WB). GTX60623

GTX60623 WB Image

WB analysis of HEK293 (1) and GCNF (AA: 65-118)-hIgGFc transfected HEK293 (2) cell lysate using GTX60623 GCNF antibody [4G8].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 409