GeneTex
United States (US)

GCP2 antibody [N1N2], N-term

GTX102281_40079_IFA_19011506_140.jpg
GTX102281_40079_WB_M_19011506_798.jpg
GTX102281_WB_19011506_572.jpg
GTX102281_40079_IHC_19011506_612.jpg

Cat No. GTX102281

Reference 1
Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
See all GCP2 products
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

103 kDa. ( Note )

Positive Control

A431 , HeLa , NIH-3T3

Predict Reactivity

Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human GCP2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Tubulin Gamma Complex Associated Protein 2,Alp4,Gcp-2,Gcp2,Grip103,Spbc97,Spc97,Spc97P,H103P,Hgcp2,Hspc97,Tubgcp2

Cellular Localization

Centrosome

Background

Gamma-tubulin complex is necessary for microtubule nucleation at the centrosome.

Database

Research Area

DATA IMAGES
GTX102281_40079_IFA_19011506_140.jpg

GTX102281 ICC/IF Image

GCP2 antibody [N1N2], N-term detects TUBGCP2 protein at centrosome by confocal immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TUBGCP2 protein stained by GCP2 antibody [N1N2], N-term (GTX102281) diluted at 1:500.
Blue: Hoechst 33342 staining.
[Images captured by Olympus FV10i Confocal Laser Scanning Microscope]
GTX102281_40079_WB_M_19011506_798.jpg

GTX102281 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
7.5% SDS PAGE
GTX102281 diluted at 1:2000

GTX102281_WB_19011506_572.jpg

GTX102281 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
B: Hela
7.5% SDS PAGE
GTX102281 diluted at 1:1000

GTX102281_40079_IHC_19011506_612.jpg

GTX102281 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using GCP2(GTX102281) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
REFERENCE

Application Reference

Draberova E et al. J Neuropathol Exp Neurol 2015; 74 (7) : 723-42 Overexpression and Nucleolar Localization of γ-Tubulin Small Complex Proteins GCP2 and GCP3 in Glioblastoma.
Submit a Reference
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169