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GCR2 antibody

Anti-GCR2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04585
Anti-GCR2 antibody used in Flow cytometry (FACS). GTX04585
Anti-GCR2 antibody used in Western Blot (WB). GTX04585

Cat. No. GTX04585

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, FACS, ELISA

Reactivity

 Human
Package
100 μg ($469)
See all GCR2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.25-0.5 μg/mL
ICC/IF 5 μg/mL
FACS 1-3 μg/10⁶ cells
ELISA 0.1-0.5 μg/mL
Not tested in other applications.

Calculated MW

73 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄

Preservative

No preservatives

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

E.coli-derived human SGT1/ECD recombinant protein (Position: L50-Q567).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ecdysoneless cell cycle regulator , GCR2 , HSGT1 , SGT1

Cellular Localization

Cytoplasm , Nucleus

Background

Enables histone acetyltransferase binding activity. Involved in positive regulation of transcription by RNA polymerase II. Located in cytosol and nucleoplasm. [provided by Alliance of Genome Resources, Apr 2022]

Database

Research Area

DATA IMAGES

Anti-GCR2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04585

GTX04585 ICC/IF Image

ICC/IF analysis of PFA fixed A431 cells using GTX04585 GCR2 antibody.
Antigen retrieval : Enzyme antigen retrieval was performed.
Green : Primary antibody
Blue : DAPI
Dillution : 5 μg/mL

Anti-GCR2 antibody used in Flow cytometry (FACS). GTX04585

GTX04585 FACS Image

FACS analysis of HeLa cells using GTX04585 GCR2 antibody.
Blue : Primary antibody
Green : Isotype control
Red : Unlabelled sample
Dilution : 1 μg/10⁶ cells

Anti-GCR2 antibody used in Western Blot (WB). GTX04585

GTX04585 WB Image

WB analysis of various cell lysates using GTX04585 GCR2 antibody.
Loading : 30 μg
Dilution : 0.5 μg/mL

REFERENCE

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REVIEW

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Package List Price ($)
$ 469