GCSF Receptor antibody [N2C1], Internal

GCSF Receptor antibody [N2C1], Internal detects GCSF Receptor protein at secreted on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
GCSF Receptor antibody [N2C1], Internal (GTX109503) diluted at 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GCSF Receptor antibody [N2C1], Internal (GTX109503) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with GCSF Receptor antibody [N2C1], Internal (GTX109503) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

GCSF Receptor antibody [N2C1], Internal detects GCSF Receptor protein at cell membrane by immunofluorescent analysis.
Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GCSF Receptor stained by GCSF Receptor antibody [N2C1], Internal (GTX109503) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).