GDF15 antibody [23B3D2.H5]

Cat. No. GTX48498
Cat. No.  GTX48498
Anti-GDF15 antibody [23B3D2.H5] used in Western Blot (WB). GTX48498
GTX48498 WB Image

WB analysis of various samples using GTX48498 GDF15 antibody [23B3D2.H5].
Lane 1 : SUMO-tagged NAG-1 H variant
Lane 2 : SUMO-tagged NAG-1 D variant
Lane 3 : NAG-1 H variant
Lane 4 : NAG-1 D variant
Dilution : 1:1000

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Anti-GDF15 antibody [23B3D2.H5] used in Western Blot (WB). GTX48498
GTX48498 WB Image

Western blot using GeneTex's anti-NAG-1 monoclonal antibody. The blot shows detection of recombinant NAG-1 protein present in Pichia pastoris whole cell lysates: lane 1 - yeast cell lysate expressing NAG-1 H variant with SUMO expression tag at 36 kDa; lane 2 - yeast cell lysate expressing NAG-1 D variant with SUMO expression tag at 36 kDa; lane 3 - yeast cell lysate expressing NAG-1 H variant; and lane 4 - yeast cell lysate expressing NAG-1 D variant. Recombinant NAG-1 proteins without SUMO correspond to monomer (15 kDa) and dimer (30 kDa) bands as indicated by the arrowheads. All lysates were run under reducing conditions. Primary antibody was used at a 1:1,000 dilution in TBS containg 1% BSA and 0.2% Tween, and reacted overnight at 4ºC. For detection, a 1:40,000 dilution of peroxidase conjugated Goat-anti-Mouse IgG secondary antibody for 30 min at room temperature. Molecular weight estimation was made by comparison to prestained MW markers.

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Anti-GDF15 antibody [23B3D2.H5] used in Western Blot (WB). GTX48498
Anti-GDF15 antibody [23B3D2.H5] used in Western Blot (WB). GTX48498
  • Host
    Mouse
  • Clonality
    Monoclonal
  • Clone Name
    23B3D2.H5
  • Isotype
    IgG1
  • Applications
    WB ELISA
  • Reactivity
    Human
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