APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.1-0.3μg/ml |
| 5μg/ml |
| 10μg/ml |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89503-PEP).
Predict Reactivity
Mouse, Rat(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-QKTDTGVSLQTYDD, from the C Terminus of the protein sequence according to NP_004855.2.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
growth differentiation factor 15 , GDF-15 , MIC-1 , MIC1 , NAG-1 , PDF , PLAB , PTGFB
Cellular Localization
Secreted
Background
This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. The protein is expressed in a broad range of cell types, acts as a pleiotropic cytokine and is involved in the stress response program of cells after cellular injury. Increased protein levels are associated with disease states such as tissue hypoxia, inflammation, acute injury and oxidative stress. [provided by RefSeq, Aug 2016]
Database
Research Area
DATA IMAGES
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GTX89503 WB Image
WB analysis of HEK293 overexpressing GDF15 (mock transfection in first lane) using GTX89503 GDF15 antibody, C-term.
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GTX89503 WB Image
WB analysis of human testes (A) and negative control skin (B) lysate using GTX89503 GDF15 antibody, C-term.
Dilution : 0.3μg/ml
Loading : 35μg protein in RIPA buffer
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GTX89503 IHC-P Image
IHC-P analysis of human prostate using GTX89503 GDF15 antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 5μg/ml
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GTX89503 FACS Image
FACS analysis of PFA fixed HeLa cells using GTX89503 GDF15 antibody, C-term.
Blue : Primary antibody
Black : Isotype control
Permeabilization : 0.5% Triton
Dilution : 10μg/ml
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GTX89503 IHC-P Image
IHC-P analysis of human placenta using GTX89503 GDF15 antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 5μg/ml
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REFERENCE
There are currently no references for GDF15 antibody, C-term (GTX89503). Be the first to share your publications with this product.
REVIEW
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