APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
NIH-3T3 , JC
Predict Reactivity
Rabbit, Bovine, Chicken, Pig, Xenopus tropicalis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human GDF5. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1950365
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
growth differentiation factor 5 , BDA1C , BMP-14 , BMP14 , CDMP1 , LAP-4 , LAP4 , OS5 , SYM1B , SYNS2
Cellular Localization
Secreted
Background
The protein encoded by this gene is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Mutations in this gene are associated with acromesomelic dysplasia, Hunter-Thompson type; brachydactyly, type C; and chondrodysplasia, Grebe type. These associations confirm that the gene product plays a role in skeletal development. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-GDF5 + GDF6 antibody [C3], C-term used in Western Blot (WB). GTX108415](/templates/default/js/lightbox/images/loading.gif "Anti-GDF5 + GDF6 antibody [C3], C-term used in Western Blot (WB). GTX108415")
|
GTX108415 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GDF5 + GDF6 antibody [C3], C-term (GTX108415) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
|
GTX108415 WB Image
Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: JC
7.5% SDS PAGE
GTX108415 diluted at 1:1000
|
|
|
GTX108415 IHC-P Image
GDF5 + GDF6 antibody [C3], C-term detects GDF5 protein at cytosol on human breast cancer by immunohistochemical analysis.
Sample: Paraffin-embedded breast cancer.
GDF5 + GDF6 antibody [C3], C-term (GTX108415) dilution: 1:250.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
|
REFERENCE
There are currently no references for GDF5 + GDF6 antibody [C3], C-term (GTX108415). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for GDF5 + GDF6 antibody [C3], C-term (GTX108415). Be the first to share your experience with this product.
![Anti-GDF5 + GDF6 antibody [C3], C-term used in Western Blot (WB). GTX108415](/upload/website/prouct_img/small/GTX108415/GTX108415_39799_20220812_WB_22081423_848.webp "Anti-GDF5 + GDF6 antibody [C3], C-term used in Western Blot (WB). GTX108415")
![Anti-GDF5 + GDF6 antibody [C3], C-term used in Western Blot (WB). GTX108415](/upload/website/prouct_img/small/GTX108415/GTX108415_39799_WB_M_w_23060120_985.webp "Anti-GDF5 + GDF6 antibody [C3], C-term used in Western Blot (WB). GTX108415")
![Anti-GDF5 + GDF6 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX108415](/upload/website/prouct_img/small/GTX108415/GTX108415_39799_IHC_w_23060120_498.webp "Anti-GDF5 + GDF6 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX108415")
![Anti-GDF5 + GDF6 antibody [C3], C-term used in Western Blot (WB). GTX108415](/upload/website/prouct_img/normal/GTX108415/GTX108415_39799_20220812_WB_22081423_848.webp "Anti-GDF5 + GDF6 antibody [C3], C-term used in Western Blot (WB). GTX108415")
![Anti-GDF5 + GDF6 antibody [C3], C-term used in Western Blot (WB). GTX108415](/upload/website/prouct_img/normal/GTX108415/GTX108415_39799_WB_M_w_23060120_985.webp "Anti-GDF5 + GDF6 antibody [C3], C-term used in Western Blot (WB). GTX108415")
![Anti-GDF5 + GDF6 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX108415](/upload/website/prouct_img/normal/GTX108415/GTX108415_39799_IHC_w_23060120_498.webp "Anti-GDF5 + GDF6 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX108415")