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GDF5 antibody

Anti-GDF5 antibody used in IHC (Paraffin sections) (IHC-P). GTX113580
Anti-GDF5 antibody used in Western Blot (WB). GTX113580

Cat No. GTX113580

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, Functional Assay

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Functional Assay Assay dependent
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

IMR32

Predict Reactivity

Mouse, Rabbit, Bovine, Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.65 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GDF5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11167042

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

growth differentiation factor 5 , BDA1C , BMP-14 , BMP14 , CDMP1 , LAP-4 , LAP4 , OS5 , SYM1B , SYNS2

Cellular Localization

Secreted

Background

The protein encoded by this gene is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Mutations in this gene are associated with acromesomelic dysplasia, Hunter-Thompson type; brachydactyly, type C; and chondrodysplasia, Grebe type. These associations confirm that the gene product plays a role in skeletal development. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GDF5 antibody used in IHC (Paraffin sections) (IHC-P). GTX113580

GTX113580 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using GDF5(GTX113580) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GDF5 antibody used in Western Blot (WB). GTX113580

GTX113580 WB Image

Sample (30 ug of whole cell lysate)
A: IMR32
7.5% SDS PAGE
GTX113580 diluted at 1:1000

REFERENCE

REVIEW

GDF5 antibody Cat No. GTX113580

Rating ( Average 5 based on 3 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
Functional Assay(Functional Assay) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : Functional Assay
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2012.PMID: 23226264
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Stem Cells Int in 2013.PMID: 24454406
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2012.PMID: 23226264
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169