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GDF8 / Myostatin antibody [13F2]

Orthogonal
Anti-GDF8 / Myostatin antibody [13F2] used in Western Blot (WB). GTX53334

Cat. No. GTX53334

Reference 1

Host

 Rat

Clonality

 Monoclonal

Clone Name

 13F2

Isotype

 IgG2

Application

 WB

Reactivity

 Mouse
Package
100 μg ($399)
See all GDF8 / Myostatin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

500 μg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Mouse recombinant protein GDF-8

Purification

Protein A/G purified
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_2887815

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Cellular Localization

Secreted

Background

This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein negatively regulates skeletal muscle cell proliferation and differentiation. Homozygous knockout mice for this gene exhibit increased muscle mass and bone density, and reduced adiposity. [provided by RefSeq, Jul 2016]

Database

Research Area

DATA IMAGES

Anti-GDF8 / Myostatin antibody [13F2] used in Western Blot (WB). GTX53334

GTX53334 WB Image

Orthogonal

The data was published in the journal PLoS One in 2015.PMID: 25875253

REFERENCE

Application Reference

Nguyen NU et al. PLoS One 2015; 10 (4) : e0124762 Dual roles of palladin protein in in vitro myogenesis: inhibition of early induction but promotion of myotube maturation.
Application : WB
Reactivity : Mouse
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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399